NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	K0E51_RS26970	Genome accession	NZ_CP080369
Coordinates	5796498..5796950 (-)	Length	150 a.a.
NCBI ID	WP_024947914.1	Uniprot ID	A4GRK7
Organism	Pseudomonas aeruginosa SG17M
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5791498..5801950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K0E51_RS26950 (K0E51_26950)	ampD	5792204..5792770 (-)	567	WP_023084738.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
K0E51_RS26955 (K0E51_26955)	-	5792921..5795176 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
K0E51_RS26960 (K0E51_26960)	nadC	5795462..5796310 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
K0E51_RS26970 (K0E51_26970)	pilA	5796498..5796950 (-)	453	WP_024947914.1	pilin	Machinery gene
K0E51_RS26975 (K0E51_26975)	pilB	5797179..5798879 (+)	1701	WP_009875880.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K0E51_RS26980 (K0E51_26980)	pilC	5798883..5800103 (+)	1221	WP_016253888.1	type 4a pilus biogenesis protein PilC	Machinery gene
K0E51_RS26985 (K0E51_26985)	pilD	5800107..5800979 (+)	873	WP_023087804.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
K0E51_RS26990 (K0E51_26990)	coaE	5800976..5801587 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
K0E51_RS26995 (K0E51_26995)	yacG	5801584..5801784 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15622.02 Da Isoelectric Point: 8.4827

                                >NTDB_id=519406 K0E51_RS26970 WP_024947914.1 5796498..5796950(-) (pilA) [Pseudomonas aeruginosa SG17M]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG

VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide

Download Length: 453 bp

                                >NTDB_id=519406 K0E51_RS26970 WP_024947914.1 5796498..5796950(-) (pilA) [Pseudomonas aeruginosa SG17M]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT

TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG

AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG

GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC

TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA

AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A4GRK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	100	100	1
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.682	100	0.48
pilA	Acinetobacter baumannii strain A118	45.333	100	0.453
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.575	97.333	0.453
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.675	100	0.447
pilA	Vibrio cholerae C6706	42.675	100	0.447
pilA	Vibrio cholerae strain A1552	42.675	100	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	48.507	89.333	0.433
comP	Acinetobacter baylyi ADP1	40.789	100	0.413
pilA2	Legionella pneumophila strain ERS1305867	39.865	98.667	0.393
pilA/pilA1	Eikenella corrodens VA1	39.456	98	0.387
pilA2	Legionella pneumophila str. Paris	39.189	98.667	0.387

Detailed information NTG0519406