Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K0E51_RS26985 Genome accession   NZ_CP080369
Coordinates   5800107..5800979 (+) Length   290 a.a.
NCBI ID   WP_023087804.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa SG17M     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5795107..5805979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0E51_RS26960 (K0E51_26960) nadC 5795462..5796310 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0E51_RS26970 (K0E51_26970) pilA 5796498..5796950 (-) 453 WP_024947914.1 pilin Machinery gene
  K0E51_RS26975 (K0E51_26975) pilB 5797179..5798879 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0E51_RS26980 (K0E51_26980) pilC 5798883..5800103 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  K0E51_RS26985 (K0E51_26985) pilD 5800107..5800979 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K0E51_RS26990 (K0E51_26990) coaE 5800976..5801587 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  K0E51_RS26995 (K0E51_26995) yacG 5801584..5801784 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  K0E51_RS27000 (K0E51_27000) - 5801821..5802030 (-) 210 WP_003094660.1 hypothetical protein -
  K0E51_RS27005 (K0E51_27005) - 5802136..5802825 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  K0E51_RS27010 (K0E51_27010) - 5802822..5803292 (-) 471 WP_003103866.1 hypothetical protein -
  K0E51_RS27015 (K0E51_27015) - 5803289..5803714 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  K0E51_RS27020 (K0E51_27020) - 5803847..5804476 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  K0E51_RS27025 (K0E51_27025) - 5804473..5804922 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  K0E51_RS27030 (K0E51_27030) - 5804948..5805118 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31869.92 Da        Isoelectric Point: 7.7068

>NTDB_id=519409 K0E51_RS26985 WP_023087804.1 5800107..5800979(+) (pilD) [Pseudomonas aeruginosa SG17M]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=519409 K0E51_RS26985 WP_023087804.1 5800107..5800979(+) (pilD) [Pseudomonas aeruginosa SG17M]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469