Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   I6H77_RS03385 Genome accession   NZ_CP066021
Coordinates   698116..699057 (-) Length   313 a.a.
NCBI ID   WP_000249583.1    Uniprot ID   A0A1X1HB40
Organism   Streptococcus oralis strain FDAARGOS_1020     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 693116..704057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H77_RS03345 (I6H77_03345) comYH 693716..694669 (-) 954 WP_000345144.1 class I SAM-dependent methyltransferase Machinery gene
  I6H77_RS03350 (I6H77_03350) - 694730..695317 (-) 588 WP_000679790.1 class I SAM-dependent methyltransferase -
  I6H77_RS03355 (I6H77_03355) comGG/cglG 695350..695763 (-) 414 WP_000265647.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6H77_RS03360 (I6H77_03360) comGF/cglF 695741..696202 (-) 462 WP_002875275.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6H77_RS03365 (I6H77_03365) comGE/cglE 696165..696467 (-) 303 WP_000413369.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6H77_RS03370 (I6H77_03370) comGD/cglD 696430..696864 (-) 435 WP_001180060.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6H77_RS03375 (I6H77_03375) comGC/cglC 696827..697150 (-) 324 WP_000735794.1 competence type IV pilus major pilin ComGC Machinery gene
  I6H77_RS03380 (I6H77_03380) comGB/cglB 697152..698168 (-) 1017 WP_002875106.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6H77_RS03385 (I6H77_03385) comGA/cglA/cilD 698116..699057 (-) 942 WP_000249583.1 competence type IV pilus ATPase ComGA Machinery gene
  I6H77_RS03390 (I6H77_03390) - 699119..699484 (-) 366 WP_000286399.1 DUF1033 family protein -
  I6H77_RS03395 (I6H77_03395) nagA 699642..700793 (-) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  I6H77_RS03400 (I6H77_03400) - 700934..702751 (-) 1818 WP_001220886.1 acyltransferase family protein -
  I6H77_RS03405 (I6H77_03405) tgt 702885..704027 (-) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35539.43 Da        Isoelectric Point: 5.4408

>NTDB_id=516462 I6H77_RS03385 WP_000249583.1 698116..699057(-) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_1020]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKKSSLRLSTVGDYRGHESLVIRLLHDEEQDLNFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIEQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=516462 I6H77_RS03385 WP_000249583.1 698116..699057(-) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_1020]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTCATTCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAGCGGTGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTGGCGGGTATGAACGTAGGAGAGAAGAGGCGCAGTCAGCTGGGTTCTTGCGACTATCAG
CATGGGGAGAAGAAGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGGCATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGATCTGAATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGACTCT
ATCTATTTGCAGGTCCAGTCGGAAGTGGCAAGACGACCTTGATGCACGAATTGGCCAAGTCTCTGTTTAAAGGGCAACAG
GTTATGTCCATTGAAGATCCTGTCGAGATCAAGCAGGAAGACATGCTCCAGTTGCAGTTGAATGAAGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGACCTCTTGATTATTGGTGAAATTCGGGATAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAGGAACACCAGCCAACTAGCTGGAATGAACAGATTGAGCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X1HB40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.179

100

0.882
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.042

0.597
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.123

85.942

0.371