Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6H87_RS10325 Genome accession   NZ_CP066018
Coordinates   2207270..2208409 (+) Length   379 a.a.
NCBI ID   WP_010815049.1    Uniprot ID   R7X966
Organism   Cupriavidus necator strain FDAARGOS_1030     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2202270..2213409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H87_RS10295 (I6H87_10295) - 2202683..2202880 (+) 198 WP_041687510.1 hypothetical protein -
  I6H87_RS10300 (I6H87_10300) - 2202974..2203459 (+) 486 WP_010815054.1 Dps family protein -
  I6H87_RS10305 (I6H87_10305) ubiA 2203601..2204458 (+) 858 WP_010815053.1 4-hydroxybenzoate octaprenyltransferase -
  I6H87_RS10310 (I6H87_10310) proC 2204558..2205394 (-) 837 WP_011615991.1 pyrroline-5-carboxylate reductase -
  I6H87_RS10315 (I6H87_10315) - 2205441..2206136 (-) 696 WP_010815051.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6H87_RS10320 (I6H87_10320) pilT 2206200..2207243 (+) 1044 WP_010815050.1 type IV pilus twitching motility protein PilT Machinery gene
  I6H87_RS10325 (I6H87_10325) pilU 2207270..2208409 (+) 1140 WP_010815049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6H87_RS10330 (I6H87_10330) - 2208482..2208976 (+) 495 WP_010815048.1 glutathione peroxidase -
  I6H87_RS10335 (I6H87_10335) - 2208983..2209672 (-) 690 WP_010815047.1 helix-turn-helix transcriptional regulator -
  I6H87_RS10340 (I6H87_10340) - 2209695..2210066 (-) 372 WP_010815046.1 VOC family protein -
  I6H87_RS10345 (I6H87_10345) - 2210152..2210877 (-) 726 WP_010815045.1 LrgB family protein -
  I6H87_RS10350 (I6H87_10350) - 2210874..2211251 (-) 378 WP_011615990.1 CidA/LrgA family protein -
  I6H87_RS10355 (I6H87_10355) glcF 2211331..2212584 (-) 1254 WP_011615989.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42069.33 Da        Isoelectric Point: 6.6384

>NTDB_id=516420 I6H87_RS10325 WP_010815049.1 2207270..2208409(+) (pilU) [Cupriavidus necator strain FDAARGOS_1030]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKIKPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPATLHEVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGKKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMVSFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSASAKDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=516420 I6H87_RS10325 WP_010815049.1 2207270..2208409(+) (pilU) [Cupriavidus necator strain FDAARGOS_1030]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATTACCTCTGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCAAGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCGCTGGGCCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAATTTCGCG
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTGTCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGCACCAT
CAATACGCGCATTCCCTCGGTGGCCGACCTGGACCTGCCGGCGACGCTGCATGAGGTGGTGATGGCCAAGCGCGGGCTGG
TGATCGTGACCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCAATGCTGGATCACCGCAACGCGCATTCGTAC
GGCCATATCATCACCATCGAGGATCCGATCGAGTACGTGCATGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGAGTCCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GTGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAT
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCCGGCAAAAAGGGGCGCGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGGCAAGATCACCTACGAGGACGCGCTACGCAATGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCGCGAGCGCCAAGGACACCGACCTGGGTGCCGGCACCG
AGCATCTCAATGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R7X966

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.348

0.578
  pilU Acinetobacter baylyi ADP1

56.825

94.723

0.538
  pilU Vibrio cholerae strain A1552

53.444

95.778

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter baumannii D1279779

42.943

87.863

0.377
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.943

87.863

0.377
  pilT Legionella pneumophila strain ERS1305867

42.643

87.863

0.375
  pilT Legionella pneumophila strain Lp02

42.643

87.863

0.375
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Neisseria meningitidis 8013

40.294

89.71

0.361