Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6G22_RS07140 Genome accession   NZ_CP065737
Coordinates   1349911..1350849 (+) Length   312 a.a.
NCBI ID   WP_031561106.1    Uniprot ID   A0AAP8DEI2
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1344911..1355849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS07140 (I6G22_07145) comGA 1349911..1350849 (+) 939 WP_031561106.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G22_RS07145 (I6G22_07150) comGB 1350743..1351816 (+) 1074 WP_074453836.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G22_RS07150 (I6G22_07155) comGC 1351944..1352213 (+) 270 WP_023188581.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G22_RS07155 (I6G22_07160) comGD 1352188..1352604 (+) 417 WP_025017137.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G22_RS07160 (I6G22_07165) comGE 1352576..1352872 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6G22_RS07165 (I6G22_07170) comGF 1352835..1353281 (+) 447 WP_032943612.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G22_RS07170 (I6G22_07175) comGG 1353320..1353604 (+) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6G22_RS07175 (I6G22_07180) - 1353694..1354131 (+) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  I6G22_RS07180 (I6G22_07185) - 1354128..1354970 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35707.88 Da        Isoelectric Point: 5.9473

>NTDB_id=513730 I6G22_RS07140 WP_031561106.1 1349911..1350849(+) (comGA) [Lactococcus lactis strain FDAARGOS_865]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=513730 I6G22_RS07140 WP_031561106.1 1349911..1350849(+) (comGA) [Lactococcus lactis strain FDAARGOS_865]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519