Detailed information NTG0513731

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: I6G22_RS07145
• Genome accession: NZ_CP065737
• Coordinates: 1350743..1351816 (+)
• Length: 357 a.a.
• NCBI ID: WP_074453836.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain FDAARGOS_865
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1345743..1356816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G22_RS07140 (I6G22_07145)	comGA	1349911..1350849 (+)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G22_RS07145 (I6G22_07150)	comGB	1350743..1351816 (+)	1074	WP_074453836.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G22_RS07150 (I6G22_07155)	comGC	1351944..1352213 (+)	270	WP_023188581.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G22_RS07155 (I6G22_07160)	comGD	1352188..1352604 (+)	417	WP_025017137.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G22_RS07160 (I6G22_07165)	comGE	1352576..1352872 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G22_RS07165 (I6G22_07170)	comGF	1352835..1353281 (+)	447	WP_032943612.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G22_RS07170 (I6G22_07175)	comGG	1353320..1353604 (+)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
I6G22_RS07175 (I6G22_07180)	-	1353694..1354131 (+)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
I6G22_RS07180 (I6G22_07185)	-	1354128..1354970 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
I6G22_RS07185 (I6G22_07190)	-	1355147..1355884 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
I6G22_RS07190 (I6G22_07195)	-	1355877..1356686 (+)	810	WP_014570791.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40340.46 Da
• Isoelectric Point: 10.1966

>NTDB_id=513731 I6G22_RS07145 WP_074453836.1 1350743..1351816(+) (comGB) [Lactococcus lactis strain FDAARGOS_865]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLEVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=513731 I6G22_RS07145 WP_074453836.1 1350743..1351816(+) (comGB) [Lactococcus lactis strain FDAARGOS_865]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTGAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.991	95.518	0.793
comYB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comYB	Streptococcus mutans UA140	53.666	95.518	0.513
comYB	Streptococcus mutans UA159	53.372	95.518	0.51
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae TIGR4	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae Rx1	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae D39	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae R6	50.746	93.838	0.476
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471