Detailed information NTG0513735

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: I6G22_RS07165
• Genome accession: NZ_CP065737
• Coordinates: 1352835..1353281 (+)
• Length: 148 a.a.
• NCBI ID: WP_032943612.1
• Uniprot ID: A0A0B8QKK0
• Organism: Lactococcus lactis strain FDAARGOS_865
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1347835..1358281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G22_RS07140 (I6G22_07145)	comGA	1349911..1350849 (+)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G22_RS07145 (I6G22_07150)	comGB	1350743..1351816 (+)	1074	WP_074453836.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G22_RS07150 (I6G22_07155)	comGC	1351944..1352213 (+)	270	WP_023188581.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G22_RS07155 (I6G22_07160)	comGD	1352188..1352604 (+)	417	WP_025017137.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G22_RS07160 (I6G22_07165)	comGE	1352576..1352872 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G22_RS07165 (I6G22_07170)	comGF	1352835..1353281 (+)	447	WP_032943612.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G22_RS07170 (I6G22_07175)	comGG	1353320..1353604 (+)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
I6G22_RS07175 (I6G22_07180)	-	1353694..1354131 (+)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
I6G22_RS07180 (I6G22_07185)	-	1354128..1354970 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
I6G22_RS07185 (I6G22_07190)	-	1355147..1355884 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
I6G22_RS07190 (I6G22_07195)	-	1355877..1356686 (+)	810	WP_014570791.1	metal ABC transporter permease	-
I6G22_RS07195 (I6G22_07200)	-	1356724..1357590 (-)	867	WP_025017140.1	RluA family pseudouridine synthase	-
I6G22_RS07200 (I6G22_07205)	-	1357795..1358172 (+)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17080.89 Da
• Isoelectric Point: 8.6780

>NTDB_id=513735 I6G22_RS07165 WP_032943612.1 1352835..1353281(+) (comGF) [Lactococcus lactis strain FDAARGOS_865]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQPMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=513735 I6G22_RS07165 WP_032943612.1 1352835..1353281(+) (comGF) [Lactococcus lactis strain FDAARGOS_865]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCCAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

3D structure

• AlphaFold DB: A0A0B8QKK0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	40.876	92.568	0.378
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378