Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   IM723_RS08980 Genome accession   NZ_CP063275
Coordinates   1741193..1742134 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain DMST-H2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1736193..1747134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM723_RS08940 - 1736269..1737462 (-) 1194 WP_014608740.1 acetate kinase -
  IM723_RS08945 comYH 1737518..1738474 (-) 957 WP_211836898.1 class I SAM-dependent methyltransferase Machinery gene
  IM723_RS08950 comGG 1738519..1738836 (-) 318 WP_141326604.1 competence type IV pilus minor pilin ComGG -
  IM723_RS08955 comYF 1738814..1739251 (-) 438 WP_022096897.1 competence type IV pilus minor pilin ComGF Machinery gene
  IM723_RS08960 comGE 1739235..1739528 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  IM723_RS08965 comYD 1739500..1739928 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  IM723_RS08970 comYC 1739888..1740214 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  IM723_RS08975 comYB 1740211..1741311 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  IM723_RS08980 comGA/cglA/cilD 1741193..1742134 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  IM723_RS08985 - 1742215..1742577 (-) 363 WP_014608746.1 DUF1033 family protein -
  IM723_RS08990 rpoC 1742736..1746374 (-) 3639 WP_211836899.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=494192 IM723_RS08980 WP_022096896.1 1741193..1742134(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain DMST-H2]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=494192 IM723_RS08980 WP_022096896.1 1741193..1742134(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain DMST-H2]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTTTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377