NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	INP95_RS00255	Genome accession	NZ_CP063117
Coordinates	53277..53720 (+)	Length	147 a.a.
NCBI ID	WP_049369499.1	Uniprot ID	-
Organism	Haemophilus parainfluenzae strain M1C142_1
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 48277..58720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
INP95_RS00225 (INP95_00225)	tadA	48491..49009 (-)	519	WP_152682592.1	tRNA adenosine(34) deaminase TadA	-
INP95_RS00230 (INP95_00230)	-	49019..49870 (-)	852	WP_049384516.1	thymidylate synthase	-
INP95_RS00235 (INP95_00235)	lgt	49884..50690 (-)	807	WP_197560667.1	prolipoprotein diacylglyceryl transferase	-
INP95_RS00240 (INP95_00240)	-	50699..51493 (-)	795	WP_070776080.1	sulfite exporter TauE/SafE family protein	-
INP95_RS00245 (INP95_00245)	rppH	51493..52086 (-)	594	WP_049384519.1	RNA pyrophosphohydrolase	-
INP95_RS00250 (INP95_00250)	ampD	52571..53134 (-)	564	WP_049371639.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
INP95_RS00255 (INP95_00255)	pilA	53277..53720 (+)	444	WP_049369499.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
INP95_RS00260 (INP95_00260)	pilB	53727..55106 (+)	1380	WP_049384520.1	GspE/PulE family protein	Machinery gene
INP95_RS00265 (INP95_00265)	pilC	55115..56338 (+)	1224	WP_049384521.1	type II secretion system F family protein	Machinery gene
INP95_RS00270 (INP95_00270)	pilD	56335..57012 (+)	678	WP_049384522.1	prepilin peptidase	Machinery gene
INP95_RS00275 (INP95_00275)	coaE	57101..57721 (+)	621	WP_049384523.1	dephospho-CoA kinase	-
INP95_RS00280 (INP95_00280)	yacG	57714..57920 (+)	207	WP_005695505.1	DNA gyrase inhibitor YacG	-
INP95_RS00285 (INP95_00285)	-	57917..58198 (+)	282	WP_049384524.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 147 a.a. Molecular weight: 15125.38 Da Isoelectric Point: 8.4674

                                >NTDB_id=493029 INP95_RS00255 WP_049369499.1 53277..53720(+) (pilA) [Haemophilus parainfluenzae strain M1C142_1]

MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCIYSTNAPTNCSGGSNGIA

ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSSAK

Nucleotide

Download Length: 444 bp

                                >NTDB_id=493029 INP95_RS00255 WP_049369499.1 53277..53720(+) (pilA) [Haemophilus parainfluenzae strain M1C142_1]

ATGAAACTCACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT

TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCAGCTATCTCCGAATTGTTACAAGCTTCTGCAC

CTTATAAATCTGATGTGGAACTATGTATCTATAGCACCAATGCCCCAACAAACTGTTCAGGTGGCTCAAATGGCATTGCA

GCAGATATCACTACCGCAAAAGGCTATGTCAAATCCATCACCACGAAATCAGGTGTGATTACGGTGACTGGAAATGGTGC

ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTTACCTGGACAACTGCTTGCCCAAGTA

ATGCTGATTTATTCCCTGCAGGCTTCTGTTCTTCTGCTAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	74.658	99.32	0.742
pilA	Haemophilus influenzae Rd KW20	72.603	99.32	0.721
pilA	Glaesserella parasuis strain SC1401	56.757	100	0.571
pilA	Vibrio campbellii strain DS40M4	41.096	99.32	0.408
pilE	Neisseria gonorrhoeae MS11	41.985	89.116	0.374
pilA2	Legionella pneumophila strain ERS1305867	42.188	87.075	0.367
pilA2	Legionella pneumophila str. Paris	42.188	87.075	0.367
pilA	Vibrio cholerae C6706	35.526	100	0.367
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	35.526	100	0.367
pilA	Vibrio cholerae strain A1552	35.526	100	0.367

Detailed information NTG0493029