Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP95_RS00260 Genome accession   NZ_CP063117
Coordinates   53727..55106 (+) Length   459 a.a.
NCBI ID   WP_049384520.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C142_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48727..60106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP95_RS00230 (INP95_00230) - 49019..49870 (-) 852 WP_049384516.1 thymidylate synthase -
  INP95_RS00235 (INP95_00235) lgt 49884..50690 (-) 807 WP_197560667.1 prolipoprotein diacylglyceryl transferase -
  INP95_RS00240 (INP95_00240) - 50699..51493 (-) 795 WP_070776080.1 sulfite exporter TauE/SafE family protein -
  INP95_RS00245 (INP95_00245) rppH 51493..52086 (-) 594 WP_049384519.1 RNA pyrophosphohydrolase -
  INP95_RS00250 (INP95_00250) ampD 52571..53134 (-) 564 WP_049371639.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP95_RS00255 (INP95_00255) pilA 53277..53720 (+) 444 WP_049369499.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  INP95_RS00260 (INP95_00260) pilB 53727..55106 (+) 1380 WP_049384520.1 GspE/PulE family protein Machinery gene
  INP95_RS00265 (INP95_00265) pilC 55115..56338 (+) 1224 WP_049384521.1 type II secretion system F family protein Machinery gene
  INP95_RS00270 (INP95_00270) pilD 56335..57012 (+) 678 WP_049384522.1 prepilin peptidase Machinery gene
  INP95_RS00275 (INP95_00275) coaE 57101..57721 (+) 621 WP_049384523.1 dephospho-CoA kinase -
  INP95_RS00280 (INP95_00280) yacG 57714..57920 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP95_RS00285 (INP95_00285) - 57917..58198 (+) 282 WP_049384524.1 GNAT family N-acetyltransferase -
  INP95_RS00290 (INP95_00290) rhlB 58289..59548 (+) 1260 WP_070776274.1 ATP-dependent RNA helicase RhlB -
  INP95_RS00295 (INP95_00295) mreD 59589..60077 (-) 489 WP_049364929.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52078.36 Da        Isoelectric Point: 6.2296

>NTDB_id=493030 INP95_RS00260 WP_049384520.1 53727..55106(+) (pilB) [Haemophilus parainfluenzae strain M1C142_1]
MAYQAIAKNGEIYHISPQYWQQNQQQQSLLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETTQLK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLANEDEPVIQLLNSIFETALQKNASDIHLETQPNGLLIRLRIDGVLQPQP
IISKALANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYRGRIGVYQFLQCENNRYQTDFDSLYQSALEKVKDHVTDLAEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=493030 INP95_RS00260 WP_049384520.1 53727..55106(+) (pilB) [Haemophilus parainfluenzae strain M1C142_1]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
ATCATTGCTGCTGCGTTACTTTGCGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCGGTGGATTCGCTGAATA
ATCTTGCGGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTGGTTGAACCGATTTTATTTGAAACGACTCAACTCAAA
CAACTTTTACAATCCCTTGCACCTAAAGCCAATCAAATCGAAGAGCAAACCACGTTTTATCATCATTCAGAAGACGAAAG
CACGGCTAATTTAGCTAATGAAGATGAACCCGTTATTCAATTATTAAACAGTATTTTTGAAACAGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAATGGGCTCTTAATTCGTTTACGTATTGATGGTGTGCTCCAACCGCAACCG
ATTATTAGTAAAGCGCTCGCCAATCGTGTAATTTCTCGCCTTAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACTACATTTTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCTACGCATT
GGGGAGAAAAAGCAGTATTACGATTACAACAAAATAAACCTGTCCAACTAAGTTTTGCTGAGTTGGGTATGACACAAAAC
CAACAAAGTCAATTTCAACATGCGCTCAGCCAACCGCAAGGTTTAATCCTGGTGACAGGCCCAACAGGAAGTGGAAAAAG
TATCTCGCTTTATACTGCACTTCAATGGCTCAACACACCAGATAAACACATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGCATCATTCAAACTCAAGTAAATCCGCAAATCGGTTTGGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTGATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCGATTTCACGCTTGCAACAACTAGGTATTCAATCTC
ACGAAATTGAAAATAGTCTCTTATTAGTTATCGCACAGAGATTGGTGCGTAAACGTTGCTTAAAGTGCGGCCAACATTTT
AGTGATTCTTGCGATTGCCATCAGGGATATCGAGGTCGTATTGGTGTGTATCAATTTTTACAATGCGAAAACAATCGCTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTAAAAGATCACGTCACCGATCTCGCCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)

Type II/IV secretion system protein (T2SSE)

(124-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.54

98.475

0.773
  pilB Haemophilus influenzae Rd KW20

77.876

98.475

0.767
  pilB Glaesserella parasuis strain SC1401

59.735

98.475

0.588
  pilB Vibrio cholerae strain A1552

41.079

100

0.431
  pilB Vibrio campbellii strain DS40M4

40.535

100

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

40.249

100

0.423
  pilB Acinetobacter baumannii D1279779

39.425

100

0.418
  pilB Acinetobacter baylyi ADP1

38.431

100

0.416
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.412
  pilF Neisseria gonorrhoeae MS11

39.394

93.464

0.368
  pilF Thermus thermophilus HB27

38.515

93.9

0.362