Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMBM01240355_RS04220 Genome accession   NC_017517
Coordinates   827322..828452 (+) Length   376 a.a.
NCBI ID   WP_041423355.1    Uniprot ID   -
Organism   Neisseria meningitidis M01-240355     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 822322..833452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBM01240355_RS15290 - 822987..823136 (+) 150 WP_019273745.1 hypothetical protein -
  NMBM01240355_RS04205 (NMBM01240355_0767) lepB 823355..824374 (-) 1020 WP_002219537.1 signal peptidase I -
  NMBM01240355_RS04210 (NMBM01240355_0768) lepA 824517..826310 (-) 1794 WP_002225433.1 translation elongation factor 4 -
  NMBM01240355_RS04215 (NMBM01240355_0769) - 826453..827154 (-) 702 WP_002251217.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMBM01240355_RS04220 (NMBM01240355_0770) pilU 827322..828452 (+) 1131 WP_041423355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMBM01240355_RS04225 (NMBM01240355_0771) - 828486..829463 (+) 978 WP_002222732.1 DNA polymerase III subunit delta' -
  NMBM01240355_RS04230 (NMBM01240355_0772) - 829467..829817 (+) 351 WP_002214009.1 PilZ domain-containing protein -
  NMBM01240355_RS04235 (NMBM01240355_0773) - 829822..830601 (+) 780 WP_014581897.1 TatD family hydrolase -
  NMBM01240355_RS04240 (NMBM01240355_0774) - 830627..830842 (+) 216 WP_002219531.1 DUF333 domain-containing protein -
  NMBM01240355_RS04245 (NMBM01240355_0775) grxD 830943..831254 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMBM01240355_RS04255 (NMBM01240355_0776) upp 831360..831986 (+) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  NMBM01240355_RS04260 (NMBM01240355_0777) - 832009..832329 (+) 321 WP_002214001.1 DUF2322 family protein -
  NMBM01240355_RS04265 (NMBM01240355_0778) - 832580..833002 (+) 423 WP_002219529.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2885

>NTDB_id=48100 NMBM01240355_RS04220 WP_041423355.1 827322..828452(+) (pilU) [Neisseria meningitidis M01-240355]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=48100 NMBM01240355_RS04220 WP_041423355.1 827322..828452(+) (pilU) [Neisseria meningitidis M01-240355]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACCCATTTCCCGCCCGCGATGAAGTTGGACGGCAAAATCACCCGCATCACGGACGAGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment