NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	EBO27_RS24740	Genome accession	NZ_CP061034
Coordinates	5298101..5298553 (-)	Length	150 a.a.
NCBI ID	WP_028681203.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain PA3
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5293101..5303553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBO27_RS24720 (EBO27_24720)	ampD	5293796..5294362 (-)	567	WP_003112844.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
EBO27_RS24725 (EBO27_24725)	-	5294513..5296768 (-)	2256	WP_187787311.1	DUF1631 domain-containing protein	-
EBO27_RS24730 (EBO27_24730)	nadC	5297054..5297902 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EBO27_RS24740 (EBO27_24740)	pilA	5298101..5298553 (-)	453	WP_028681203.1	pilin	Machinery gene
EBO27_RS24745 (EBO27_24745)	pilB	5298785..5300485 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EBO27_RS24750 (EBO27_24750)	pilC	5300489..5301709 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
EBO27_RS24755 (EBO27_24755)	pilD	5301713..5302585 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
EBO27_RS24760 (EBO27_24760)	coaE	5302582..5303193 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
EBO27_RS24765 (EBO27_24765)	yacG	5303190..5303390 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15671.99 Da Isoelectric Point: 7.7813

                                >NTDB_id=479952 EBO27_RS24740 WP_028681203.1 5298101..5298553(-) (pilA) [Pseudomonas aeruginosa strain PA3]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=479952 EBO27_RS24740 WP_028681203.1 5298101..5298553(-) (pilA) [Pseudomonas aeruginosa strain PA3]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	45	100	0.48
pilA	Vibrio cholerae strain A1552	45	100	0.48
pilA	Vibrio cholerae C6706	45	100	0.48
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46	100	0.46
pilA	Acinetobacter baumannii strain A118	45.27	98.667	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	42.568	98.667	0.42
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.958	95.333	0.4
pilA2	Legionella pneumophila strain ERS1305867	38.926	99.333	0.387
pilA2	Legionella pneumophila str. Paris	38.255	99.333	0.38
pilA	Acinetobacter nosocomialis M2	42.537	89.333	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	39.583	96	0.38

Detailed information NTG0479952