Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMBB_RS04190 Genome accession   NC_017505
Coordinates   801301..802431 (+) Length   376 a.a.
NCBI ID   WP_012221459.1    Uniprot ID   -
Organism   Neisseria meningitidis alpha710     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796301..807431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBB_RS04175 (NMBB_0864) lepB 797334..798353 (-) 1020 WP_002219537.1 signal peptidase I -
  NMBB_RS04180 (NMBB_0865) lepA 798496..800289 (-) 1794 WP_014575259.1 translation elongation factor 4 -
  NMBB_RS04185 (NMBB_0867) - 800432..801133 (-) 702 WP_014575260.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMBB_RS04190 (NMBB_0868) pilU 801301..802431 (+) 1131 WP_012221459.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMBB_RS04195 (NMBB_0869) - 802465..803442 (+) 978 WP_002223913.1 DNA polymerase III subunit delta' -
  NMBB_RS04200 (NMBB_0870) - 803446..803796 (+) 351 WP_002214009.1 PilZ domain-containing protein -
  NMBB_RS04205 (NMBB_0871) - 803801..804580 (+) 780 WP_002219532.1 TatD family hydrolase -
  NMBB_RS04210 - 804606..804821 (+) 216 WP_002219531.1 DUF333 domain-containing protein -
  NMBB_RS04215 (NMBB_0873) grxD 804922..805233 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMBB_RS04225 (NMBB_0875) upp 805339..805965 (+) 627 WP_002219530.1 uracil phosphoribosyltransferase -
  NMBB_RS04230 (NMBB_0876) - 805988..806308 (+) 321 WP_002214001.1 DUF2322 family protein -
  NMBB_RS04235 (NMBB_0878) - 806559..806981 (+) 423 WP_002219529.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2885

>NTDB_id=47785 NMBB_RS04190 WP_012221459.1 801301..802431(+) (pilU) [Neisseria meningitidis alpha710]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=47785 NMBB_RS04190 WP_012221459.1 801301..802431(+) (pilU) [Neisseria meningitidis alpha710]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACCCATTTCCCGCCCGCGATGAAGTTGGACGGCAAAATCACCCGCATCACGGACGAGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCTTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTGCAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.943

93.617

0.561
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.648

94.415

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment