Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMBB_RS00245 Genome accession   NC_017505
Coordinates   42309..43574 (+) Length   421 a.a.
NCBI ID   WP_002220091.1    Uniprot ID   X5EMG2
Organism   Neisseria meningitidis alpha710     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 37309..48574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBB_RS00225 (NMBB_0046) - 38039..39040 (+) 1002 WP_002220087.1 thiamine ABC transporter substrate-binding protein -
  NMBB_RS00230 (NMBB_0047) - 39134..39982 (+) 849 WP_002215243.1 mechanosensitive ion channel family protein -
  NMBB_RS00235 (NMBB_0048) - 40009..40494 (+) 486 WP_002215245.1 CinA family protein -
  NMBB_RS00240 (NMBB_0049) msrAB 40596..42164 (-) 1569 WP_002216163.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  NMBB_RS00245 (NMBB_0050) pilA 42309..43574 (+) 1266 WP_002220091.1 signal recognition particle-docking protein FtsY Machinery gene
  NMBB_RS00255 (NMBB_0053) pilC 44302..47466 (+) 3165 WP_014575084.1 PilC family type IV pilus tip adhesin Machinery gene

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45189.45 Da        Isoelectric Point: 4.5275

>NTDB_id=47769 NMBB_RS00245 WP_002220091.1 42309..43574(+) (pilA) [Neisseria meningitidis alpha710]
MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAESLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAE
EAAEKAAEQVEAAKEAIAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPFVIMLAGINGAGKTTSIGK
LAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNNVTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHL
MEEIKKVKRVLQKAMPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVRYIGV
GEGIDDLRPFDARAFVDALLD

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=47769 NMBB_RS00245 WP_002220091.1 42309..43574(+) (pilA) [Neisseria meningitidis alpha710]
ATGTTCAGCTTCTTCCGTCGCAAGAAAAAACAGGAAACGCCGGCTCTCGAGGAGGCTCAAATTCAGGAAACCGCAGCAAA
AGCAGAGTCCGAAACCGATCAAGTAATTGAAAATATTAAAGAAGATGCTGAATCTTTAGCAGAAAGCGTCAAAGGGCAGG
TCGAATCTGCCGTTGAAACCGTCAGCGGTGCGGTTGAACAGGTAAAGGAAACCGTTGCCGAGATGCTGTCTGAAGCAGAG
GAAGCGGCGGAAAAAGCAGCGGAACAAGTCGAAGCGGCAAAAGAAGCCATTGCCGAAACCGTCGGCGAGGCTGTCGGGCA
AGTTCAAGAAGCCGTTGCGACAACTGAAGAACACAAGCTCGGTTGGGCGGCGCGTTTGAAACAAGGCCTGACCAAATCGC
GCGACAAAATGGCGAAATCGCTGGCGGGCGTGTTCGGCGGCGGACAAATCGACGAAGATTTGTACGAAGAGCTGGAAACC
GTGCTAATTACCAGCGACATGGGCATGGAAGCCACCGAATACCTGATGAAAGACGTGCGCGACCGCGTCAGCCTCAAAGG
GCTGAAAGACGGCAACGAATTGCGCGGCGCGTTGAAAGAAGCCTTGTACGACCTGATTAAGCCTCTGGAAAAACCATTGG
TCTTGCCCGAAACCAAAGAGCCGTTTGTCATCATGCTTGCCGGCATCAACGGCGCGGGCAAAACCACGTCTATCGGCAAA
CTCGCCAAATATTTCCAAGCGCAGGGCAAATCCGTATTGCTGGCGGCAGGCGACACTTTCCGCGCCGCCGCCCGTGAGCA
GCTTCAGGCATGGGGCGAACGCAACAACGTTACCGTGATTTCGCAAACCACGGGCGATTCCGCCGCCGTGTGCTTCGATG
CCGTCCAAGCCGCCAAAGCGCGCGGCATCGACATTGTGCTGGCCGACACCGCCGGCCGCCTGCCCACGCAGCTTCATTTG
ATGGAAGAAATCAAAAAAGTGAAACGCGTGCTGCAAAAAGCCATGCCCGACGCGCCGCACGAAATCATCGTCGTGCTTGA
TGCCAACATCGGGCAAAACGCCGTCAACCAAGTCAAAGCCTTTGACGACGCATTAGGGCTGACCGGTTTAATCGTGACCA
AACTCGACGGCACGGCAAAAGGCGGCATCCTCGCCGCGCTTGCCTCCGACCGCCCCGTCCCCGTCCGCTATATCGGCGTG
GGCGAAGGCATAGACGATTTGCGCCCGTTTGACGCGCGCGCGTTTGTGGACGCACTGCTGGATTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(221-420)

SRP54-type protein, helical bundle domain (SRP54_N)

(136-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB X5EMG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

93.824

100

0.938

Multiple sequence alignment