Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSBA01_RS04460 Genome accession   NZ_CP060390
Coordinates   1052478..1053599 (+) Length   373 a.a.
NCBI ID   WP_071002369.1    Uniprot ID   A0A1G6U944
Organism   Psychrobacter sp. KCTC 72983     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047478..1058599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSBA01_RS04445 - 1048834..1050108 (+) 1275 WP_186475165.1 hemolysin family protein -
  PSBA01_RS04450 - 1050209..1050937 (-) 729 WP_186475437.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSBA01_RS04455 pilT 1051303..1052358 (+) 1056 WP_058024233.1 type IV pilus twitching motility protein PilT Machinery gene
  PSBA01_RS04460 pilU 1052478..1053599 (+) 1122 WP_071002369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSBA01_RS04465 fur 1053736..1054170 (-) 435 WP_058024231.1 ferric iron uptake transcriptional regulator -
  PSBA01_RS04470 - 1054498..1054917 (+) 420 WP_058024230.1 outer membrane protein assembly factor BamE -
  PSBA01_RS04475 - 1054968..1055345 (-) 378 WP_228724635.1 RnfH family protein -
  PSBA01_RS04480 - 1055321..1056451 (-) 1131 WP_186475166.1 hypothetical protein -
  PSBA01_RS04485 - 1056765..1057433 (-) 669 WP_075107765.1 c-type cytochrome -
  PSBA01_RS04490 - 1057757..1058092 (-) 336 WP_058024226.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41619.92 Da        Isoelectric Point: 7.1147

>NTDB_id=475613 PSBA01_RS04460 WP_071002369.1 1052478..1053599(+) (pilU) [Psychrobacter sp. KCTC 72983]
MDIDKLLQLMINKNGSDLFITADVAPSMKVNGKILPVGKTPLTADQTMMLVKGVMTPSQQKEFKETNECQFAISDEAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSSTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSKNGKANLDEGADSLSLDIS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=475613 PSBA01_RS04460 WP_071002369.1 1052478..1053599(+) (pilU) [Psychrobacter sp. KCTC 72983]
ATGGACATTGATAAATTATTGCAGTTGATGATTAATAAAAATGGCTCTGACCTTTTTATTACTGCTGATGTAGCGCCTTC
CATGAAAGTAAATGGAAAAATTTTACCTGTCGGTAAAACCCCTTTGACCGCTGATCAAACCATGATGTTAGTCAAAGGAG
TGATGACCCCTAGCCAGCAAAAAGAATTTAAAGAGACGAACGAGTGTCAGTTTGCTATATCTGATGAAGCACAGCAAGCA
CGTTTTCGGGTCAGTGCATTCATACAGCGCGATATGGCAGGAATGATTTTACGGAAAATTGAGAATAAGATCCCAACTGT
TGAGGAACTACGCTTGCCGCCAGCGCTCAAAGAACTGGCTATGAAAAAGCGCGGCATTATCTTGCTGGTCGGCGCGACCG
GCACAGGTAAATCCTCTACGCTCGCGGCTATGATTGGACATCGTAACCAAAACTCTCATGGGCATATCATTACCATAGAA
GACCCTATCGAGTTTGTCCATAAACATCGTGGTTGTATCATCACTCAGCGTGAAGTCGGTATCGACACTCATTCATTTGA
AGCAGGACTAAAAAATACGTTGCGCCAAGCACCAGATGTCATCTTGATTGGTGAGATTCGTAACCGTGAGGTCATGAGCT
ACGCTATCCAGTACGCTGAGACAGGGCATCTGGTATTTGCAACCTTGCATGCCAATAATGCCAACCAAGCGATTGACCGT
ATTATTCATTTCTTTGAAACCAGTCGTCATAATCAGCTATTTATGGATTTGTCACTGAACTTGCAGGCCATTATCGCTCA
GCAGCTCATTCCAACCCCTGATGGCAAAGGACGCCGTGCTGCTATTGAGATTTTATTAAACTCACAGCTAATTAGCGACT
ATATCCGTAAAGGTGAGGTGCATGAAATCAAAGACGTCATGACACGCTCTCGAGACAGCGGTATGCAAACCTTTGATCAA
GCGCTTTTTGACTTGTATGAACAAGGAGAGATCACTTATAAAGAGGCAATTTTGCATGCTGACTCCCCTAACGACTTACG
TCTGAAAATTAAGCTCAGTAGCAAAAACGGTAAGGCTAATTTAGATGAAGGTGCGGATAGTTTATCTTTAGACATCAGTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G6U944

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.488

100

0.665
  pilU Acinetobacter baylyi ADP1

66.48

95.979

0.638
  pilU Vibrio cholerae strain A1552

52.717

98.66

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.53

98.123

0.408
  pilT Legionella pneumophila strain Lp02

40.774

90.08

0.367
  pilT Legionella pneumophila strain ERS1305867

40.774

90.08

0.367
  pilT Acinetobacter nosocomialis M2

39.65

91.957

0.365
  pilT Acinetobacter baumannii strain A118

39.65

91.957

0.365
  pilT Acinetobacter baumannii D1279779

39.65

91.957

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.299

89.812

0.362
  pilT Vibrio cholerae strain A1552

40.299

89.812

0.362
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362