Detailed information NTG0470762

Bioinformatically predicted

Overview

• Name: pilA/pilA1
• Type: Machinery gene
• Locus tag: H3L94_RS00755
• Genome accession: NZ_CP059567
• Coordinates: 166781..167221 (+)
• Length: 146 a.a.
• NCBI ID: WP_182122265.1
• Uniprot ID: A0A7D7S8B5
• Organism: Neisseria shayeganii strain DSM 22244
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 161781..172221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H3L94_RS00725 (H3L94_00725)	-	162574..163065 (+)	492	WP_009119895.1	DUF456 family protein	-
H3L94_RS12185	-	163025..163258 (-)	234	WP_244958856.1	signal peptidase	-
H3L94_RS00730 (H3L94_00730)	-	163277..163690 (+)	414	WP_009119894.1	PTS mannose transporter subunit IIA	-
H3L94_RS00735 (H3L94_00735)	-	163684..163953 (+)	270	WP_009119893.1	HPr family phosphocarrier protein	-
H3L94_RS00740 (H3L94_00740)	ptsP	163956..165707 (+)	1752	WP_182122263.1	phosphoenolpyruvate--protein phosphotransferase	-
H3L94_RS00745 (H3L94_00745)	-	165857..166099 (+)	243	WP_009119890.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H3L94_RS00750 (H3L94_00750)	-	166089..166376 (+)	288	WP_182122264.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H3L94_RS00755 (H3L94_00755)	pilA/pilA1	166781..167221 (+)	441	WP_182122265.1	pilin	Machinery gene
H3L94_RS00760 (H3L94_00760)	pilA/pilA1	167299..167769 (+)	471	WP_182122266.1	pilin	Machinery gene
H3L94_RS00765 (H3L94_00765)	tfpZ	167777..168517 (+)	741	WP_182122267.1	TfpX/TfpZ family type IV pilin accessory protein	-
H3L94_RS00770 (H3L94_00770)	ccsA	168600..169409 (-)	810	WP_182122268.1	cytochrome c biogenesis protein CcsA	-
H3L94_RS00775 (H3L94_00775)	ffh	169738..171099 (+)	1362	WP_182122269.1	signal recognition particle protein	-
H3L94_RS00780 (H3L94_00780)	-	171319..171660 (+)	342	WP_182122270.1	hypothetical protein	-
H3L94_RS12200	-	171676..171840 (+)	165	Protein_159	ATP-dependent exonuclease	-

Sequence

Protein

• Length: 146 a.a.
• Molecular weight: 14677.72 Da
• Isoelectric Point: 6.9425

>NTDB_id=470762 H3L94_RS00755 WP_182122265.1 166781..167221(+) (pilA/pilA1) [Neisseria shayeganii strain DSM 22244]
MLKQVQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQATEGFKATSGLQTDIGVVLADTGSYPAANSAVHTAAAALD
GKYFNAGNVSVGDGTGVISVAFTAGANSGKGMTITPAQSNGQITGWTCAPAAANGIEAKRLPSSCQ

Nucleotide

• Length: 441 bp

>NTDB_id=470762 H3L94_RS00755 WP_182122265.1 166781..167221(+) (pilA/pilA1) [Neisseria shayeganii strain DSM 22244]
ATGTTGAAACAAGTACAAAAAGGTTTTACCTTGATCGAGCTGATGATCGTGATCGCCATTATCGGTATTCTGGCCGCCAT
CGCCCTGCCGGCTTACCAAGATTACACCGCCCGCGCTCAAGCTACTGAAGGCTTTAAAGCCACTTCCGGTTTGCAAACTG
ATATTGGTGTAGTGTTGGCTGATACTGGCTCTTATCCTGCTGCTAATAGCGCTGTACATACTGCTGCTGCTGCGTTGGAT
GGCAAATATTTCAATGCAGGTAATGTAAGTGTTGGCGATGGTACTGGTGTTATTTCAGTTGCTTTTACTGCGGGTGCTAA
TAGCGGTAAAGGCATGACCATCACTCCGGCTCAATCAAACGGCCAGATTACTGGCTGGACTTGCGCCCCTGCAGCTGCCA
ATGGTATTGAGGCCAAACGTCTGCCTTCCAGCTGTCAATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (37-146)

• Prokaryotic N-terminal methylation motif (N_methyl) (5-29)

3D structure

• AlphaFold DB: A0A7D7S8B5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilA1	Eikenella corrodens VA1	68.627	100	0.719
pilA	Ralstonia pseudosolanacearum GMI1000	37.87	100	0.438
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.105	100	0.438
pilE	Neisseria gonorrhoeae MS11	38.65	100	0.432
pilA2	Legionella pneumophila str. Paris	43.357	97.945	0.425
pilA2	Legionella pneumophila strain ERS1305867	42.254	97.26	0.411
comP	Acinetobacter baylyi ADP1	40.268	100	0.411
pilE	Neisseria gonorrhoeae strain FA1090	36.478	100	0.397
pilA	Pseudomonas aeruginosa PAK	38.571	95.89	0.37
pilA	Vibrio cholerae C6706	37.589	96.575	0.363
pilA	Vibrio cholerae strain A1552	37.589	96.575	0.363
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.589	96.575	0.363