Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   H3L94_RS00760 Genome accession   NZ_CP059567
Coordinates   167299..167769 (+) Length   156 a.a.
NCBI ID   WP_182122266.1    Uniprot ID   A0A7D7SH79
Organism   Neisseria shayeganii strain DSM 22244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162299..172769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L94_RS00725 (H3L94_00725) - 162574..163065 (+) 492 WP_009119895.1 DUF456 family protein -
  H3L94_RS12185 - 163025..163258 (-) 234 WP_244958856.1 signal peptidase -
  H3L94_RS00730 (H3L94_00730) - 163277..163690 (+) 414 WP_009119894.1 PTS mannose transporter subunit IIA -
  H3L94_RS00735 (H3L94_00735) - 163684..163953 (+) 270 WP_009119893.1 HPr family phosphocarrier protein -
  H3L94_RS00740 (H3L94_00740) ptsP 163956..165707 (+) 1752 WP_182122263.1 phosphoenolpyruvate--protein phosphotransferase -
  H3L94_RS00745 (H3L94_00745) - 165857..166099 (+) 243 WP_009119890.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  H3L94_RS00750 (H3L94_00750) - 166089..166376 (+) 288 WP_182122264.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H3L94_RS00755 (H3L94_00755) pilA/pilA1 166781..167221 (+) 441 WP_182122265.1 pilin Machinery gene
  H3L94_RS00760 (H3L94_00760) pilA/pilA1 167299..167769 (+) 471 WP_182122266.1 pilin Machinery gene
  H3L94_RS00765 (H3L94_00765) tfpZ 167777..168517 (+) 741 WP_182122267.1 TfpX/TfpZ family type IV pilin accessory protein -
  H3L94_RS00770 (H3L94_00770) ccsA 168600..169409 (-) 810 WP_182122268.1 cytochrome c biogenesis protein CcsA -
  H3L94_RS00775 (H3L94_00775) ffh 169738..171099 (+) 1362 WP_182122269.1 signal recognition particle protein -
  H3L94_RS00780 (H3L94_00780) - 171319..171660 (+) 342 WP_182122270.1 hypothetical protein -
  H3L94_RS12200 - 171676..171840 (+) 165 Protein_159 ATP-dependent exonuclease -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16371.78 Da        Isoelectric Point: 4.3466

>NTDB_id=470763 H3L94_RS00760 WP_182122266.1 167299..167769(+) (pilA/pilA1) [Neisseria shayeganii strain DSM 22244]
MKKSSGFTLIELMIVIAIIGILAALALPAYQNYTGRAQTMEGFSATEGLRQEIAIWLANYKAFPDAAAVAATGYIGQQAA
ALQGKYIDDGGITVAADSGVITVPFDAGTISGLELVLTPTVNPRANLNEQVIMWVCSANSTTLSNPERFIPSSCAN

Nucleotide


Download         Length: 471 bp        

>NTDB_id=470763 H3L94_RS00760 WP_182122266.1 167299..167769(+) (pilA/pilA1) [Neisseria shayeganii strain DSM 22244]
ATGAAAAAATCCAGCGGTTTTACCTTGATTGAGCTGATGATTGTGATTGCCATCATCGGAATCTTGGCGGCGCTTGCACT
GCCGGCTTATCAAAACTACACCGGCCGCGCACAAACCATGGAAGGTTTCTCGGCAACCGAGGGATTGCGGCAGGAGATAG
CCATTTGGCTGGCCAATTATAAAGCTTTCCCTGATGCGGCAGCGGTAGCGGCTACCGGCTATATCGGCCAGCAGGCGGCG
GCTTTGCAAGGGAAGTATATTGATGACGGCGGCATTACGGTGGCGGCGGATAGTGGTGTGATTACCGTGCCGTTTGATGC
GGGAACTATCTCTGGATTGGAATTGGTGCTCACGCCCACTGTTAATCCAAGGGCTAATTTAAACGAACAGGTAATTATGT
GGGTGTGTAGTGCCAACAGCACCACTTTGAGTAACCCCGAGCGCTTTATCCCGTCTTCTTGCGCCAACTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D7SH79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

49.02

98.077

0.481