Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ToN1_RS09215 Genome accession   NZ_CP059560
Coordinates   2014962..2015381 (+) Length   139 a.a.
NCBI ID   WP_169206171.1    Uniprot ID   -
Organism   Aromatoleum petrolei strain ToN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2009962..2020381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ToN1_RS09210 (ToN1_18890) - 2011538..2014702 (+) 3165 WP_169206170.1 efflux RND transporter permease subunit -
  ToN1_RS09215 (ToN1_18900) pilA2 2014962..2015381 (+) 420 WP_169206171.1 pilin Machinery gene
  ToN1_RS09220 (ToN1_18910) - 2015468..2017219 (+) 1752 WP_169206172.1 O-antigen ligase family protein -
  ToN1_RS09225 (ToN1_18920) pilR 2017338..2018729 (-) 1392 WP_169206173.1 sigma-54 dependent transcriptional regulator Regulator
  ToN1_RS09230 (ToN1_18930) - 2018726..2020294 (-) 1569 WP_169206174.1 ATP-binding protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14216.55 Da        Isoelectric Point: 8.4744

>NTDB_id=470623 ToN1_RS09215 WP_169206171.1 2014962..2015381(+) (pilA2) [Aromatoleum petrolei strain ToN1]
MKKVQQGFTLIELMIVVAIIGILAAVALPAYQDYTTRAKVSEVIVMSAPAKLAVSETASSLGSLALVTASNSGYTFPGAT
KYVSNIAIADTTGVVTVTSTVPGATGDLTMTPTVVGGGQLKWACASTAINSKFLPSECR

Nucleotide


Download         Length: 420 bp        

>NTDB_id=470623 ToN1_RS09215 WP_169206171.1 2014962..2015381(+) (pilA2) [Aromatoleum petrolei strain ToN1]
ATGAAAAAGGTTCAACAAGGCTTTACCCTGATCGAACTGATGATCGTCGTGGCGATTATCGGTATTTTGGCTGCCGTCGC
ACTGCCCGCGTATCAGGATTACACGACCCGGGCAAAGGTCAGCGAAGTCATCGTTATGAGCGCCCCTGCCAAACTTGCTG
TGTCGGAAACGGCGTCGTCCCTTGGATCACTTGCCCTTGTAACCGCATCTAATAGCGGATACACCTTCCCGGGCGCTACG
AAGTACGTCAGTAACATCGCGATTGCCGATACCACCGGCGTTGTCACCGTCACCTCGACGGTGCCGGGCGCAACAGGCGA
CCTTACGATGACGCCGACGGTTGTTGGCGGCGGTCAACTGAAATGGGCCTGCGCATCTACTGCAATCAACTCCAAGTTCC
TCCCCTCCGAATGCCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.111

97.122

0.496
  pilA2 Legionella pneumophila strain ERS1305867

51.111

97.122

0.496
  pilA Ralstonia pseudosolanacearum GMI1000

41.212

100

0.489
  pilE Neisseria gonorrhoeae strain FA1090

40.741

100

0.475
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.043

100

0.46
  comP Acinetobacter baylyi ADP1

44.138

100

0.46
  pilE Neisseria gonorrhoeae MS11

39.506

100

0.46
  pilA/pilA1 Eikenella corrodens VA1

39.474

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.667

100

0.432
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.161

100

0.403
  pilA Pseudomonas aeruginosa PAK

34.416

100

0.381
  pilA Acinetobacter baumannii strain A118

36.806

100

0.381
  comF Acinetobacter baylyi ADP1

35.172

100

0.367