NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	pbN1_RS06280	Genome accession	NZ_CP059467
Coordinates	1324211..1324630 (+)	Length	139 a.a.
NCBI ID	WP_169204301.1	Uniprot ID	-
Organism	Aromatoleum bremense strain PbN1
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1319211..1329630

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
pbN1_RS06255 (pbN1_12690)	-	1319407..1319880 (+)	474	WP_169204145.1	RES family NAD+ phosphorylase	-
pbN1_RS06260 (pbN1_12700)	-	1319886..1320959 (-)	1074	WP_169204146.1	tellurite resistance/C4-dicarboxylate transporter family protein	-
pbN1_RS06265 (pbN1_12720)	-	1321263..1321601 (+)	339	WP_169204147.1	nucleotidyltransferase domain-containing protein	-
pbN1_RS06270 (pbN1_12730)	-	1321647..1323158 (-)	1512	WP_169204148.1	PHB depolymerase family esterase	-
pbN1_RS06275 (pbN1_12740)	-	1323361..1323924 (-)	564	WP_210147678.1	SEL1-like repeat protein	-
pbN1_RS06280 (pbN1_12750)	pilA	1324211..1324630 (+)	420	WP_169204301.1	pilin	Machinery gene
pbN1_RS06285 (pbN1_12760)	-	1324794..1325663 (+)	870	WP_169204302.1	alkaline phosphatase family protein	-
pbN1_RS06290 (pbN1_12770)	-	1325700..1326869 (+)	1170	WP_169204314.1	transposase	-
pbN1_RS06295 (pbN1_12780)	-	1326989..1328155 (+)	1167	WP_169202982.1	sulfatase-like hydrolase/transferase	-
pbN1_RS06300	-	1328262..1328618 (+)	357	WP_244857180.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
pbN1_RS06305 (pbN1_12790)	-	1328564..1328902 (-)	339	WP_169202981.1	nucleotidyltransferase domain-containing protein	-
pbN1_RS06310 (pbN1_12800)	-	1328964..1329560 (-)	597	WP_244857181.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 139 a.a. Molecular weight: 14109.29 Da Isoelectric Point: 8.8227

                                >NTDB_id=469753 pbN1_RS06280 WP_169204301.1 1324211..1324630(+) (pilA) [Aromatoleum bremense strain PbN1]

MKKVQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSELAIMASGAKATVGENIANKGGTIDGTACNGVAVFSTAT

KNTASLACANGTITVTGTADAKAVILTYAPSSASAGITWKCSSAATNYKYVPAECRQAA

Nucleotide

Download Length: 420 bp

                                >NTDB_id=469753 pbN1_RS06280 WP_169204301.1 1324211..1324630(+) (pilA) [Aromatoleum bremense strain PbN1]

ATGAAAAAGGTGCAACAAGGTTTCACGCTGATCGAGCTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGGCGGTGGC

GCTGCCGGCGTATCAGGATTACACGGTTCGTGCCAGAGTATCCGAACTAGCCATTATGGCATCTGGCGCAAAAGCAACCG

TGGGTGAAAACATCGCGAACAAGGGGGGCACGATCGACGGCACTGCTTGCAACGGTGTTGCCGTGTTCAGCACGGCAACA

AAAAACACCGCATCTCTTGCCTGCGCGAACGGCACGATCACAGTGACCGGCACCGCCGACGCGAAAGCCGTGATTTTGAC

GTATGCTCCGTCGTCGGCGTCCGCAGGTATTACTTGGAAGTGCTCGTCAGCGGCGACAAATTACAAATACGTGCCTGCGG

AATGTCGCCAAGCCGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-136)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Ralstonia pseudosolanacearum GMI1000	48.485	100	0.576
comP	Acinetobacter baylyi ADP1	52.778	100	0.547
pilA2	Legionella pneumophila str. Paris	50	96.403	0.482
pilA2	Legionella pneumophila strain ERS1305867	49.254	96.403	0.475
pilA/pilA1	Eikenella corrodens VA1	39.216	100	0.432
pilE	Neisseria gonorrhoeae MS11	37.037	100	0.432
pilE	Neisseria gonorrhoeae strain FA1090	37.267	100	0.432
pilA	Pseudomonas aeruginosa PAK	36.478	100	0.417
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.86	100	0.41
pilA	Haemophilus influenzae Rd KW20	39.568	100	0.396
pilA	Glaesserella parasuis strain SC1401	35.526	100	0.388
pilA	Haemophilus influenzae 86-028NP	38.971	97.842	0.381
pilA	Vibrio parahaemolyticus RIMD 2210633	37.063	100	0.381

Detailed information NTG0469753