Detailed information NTG0046471

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HMPREF0772_RS08340
• Genome accession: NC_017342
• Coordinates: 1648744..1649430 (+)
• Length: 228 a.a.
• NCBI ID: WP_001794049.1
• Uniprot ID: A0A0E1X4U4
• Organism: Staphylococcus aureus subsp. aureus TCH60
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1643744..1654430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF0772_RS08305 (HMPREF0772_11544)	yqeH	1644271..1645371 (+)	1101	WP_001280133.1	ribosome biogenesis GTPase YqeH	-
HMPREF0772_RS08310 (HMPREF0772_11545)	aroE	1645385..1646191 (+)	807	WP_000666750.1	shikimate dehydrogenase	-
HMPREF0772_RS08315 (HMPREF0772_11546)	yhbY	1646195..1646485 (+)	291	WP_000955236.1	ribosome assembly RNA-binding protein YhbY	-
HMPREF0772_RS08320 (HMPREF0772_11547)	nadD	1646488..1647057 (+)	570	WP_000822938.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
HMPREF0772_RS08325 (HMPREF0772_11548)	yqeK	1647047..1647631 (+)	585	WP_001019324.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HMPREF0772_RS08330 (HMPREF0772_11549)	rsfS	1647632..1647985 (+)	354	WP_001088020.1	ribosome silencing factor	-
HMPREF0772_RS08335 (HMPREF0772_11550)	-	1647988..1648704 (+)	717	WP_000084835.1	class I SAM-dependent methyltransferase	-
HMPREF0772_RS08340 (HMPREF0772_11551)	comEA	1648744..1649430 (+)	687	WP_001794049.1	ComEA family DNA-binding protein	Machinery gene
HMPREF0772_RS08345 (HMPREF0772_11552)	-	1649522..1649983 (+)	462	WP_000439692.1	ComE operon protein 2	-
HMPREF0772_RS08350 (HMPREF0772_11553)	comEC	1649988..1652189 (+)	2202	WP_000967506.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HMPREF0772_RS08355 (HMPREF0772_11554)	holA	1652246..1653220 (+)	975	WP_001282571.1	DNA polymerase III subunit delta	-
HMPREF0772_RS08360 (HMPREF0772_11555)	rpsT	1653265..1653516 (-)	252	WP_001274017.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 25766.38 Da
• Isoelectric Point: 9.3383

>NTDB_id=46471 HMPREF0772_RS08340 WP_001794049.1 1648744..1649430(+) (comEA) [Staphylococcus aureus subsp. aureus TCH60]
MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLNSYFTT

Nucleotide

• Length: 687 bp

>NTDB_id=46471 HMPREF0772_RS08340 WP_001794049.1 1648744..1649430(+) (comEA) [Staphylococcus aureus subsp. aureus TCH60]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAGCTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
TTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAATTCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (164-227)

3D structure

• AlphaFold DB: A0A0E1X4U4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Staphylococcus aureus MW2	95.595	99.561	0.952
comEA	Staphylococcus aureus N315	95.595	99.561	0.952