Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCVT_RS02495 Genome accession   NZ_CP053826
Coordinates   519764..520885 (+) Length   373 a.a.
NCBI ID   WP_011991975.1    Uniprot ID   A7GX44
Organism   Campylobacter curvus strain ATCC 35224     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514764..525885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCVT_RS02460 (CCVT_0484) - 515587..516228 (+) 642 WP_018136897.1 DUF6803 family protein -
  CCVT_RS02465 (CCVT_0485) hisG 516222..516842 (-) 621 WP_011991971.1 ATP phosphoribosyltransferase -
  CCVT_RS02470 (CCVT_0486) - 516839..517468 (-) 630 WP_018136896.1 type III pantothenate kinase -
  CCVT_RS02475 (CCVT_0487) - 517455..517787 (-) 333 WP_009650179.1 hypothetical protein -
  CCVT_RS02480 (CCVT_0488) - 517787..518791 (-) 1005 WP_018136895.1 L-seryl-tRNA selenium transferase -
  CCVT_RS02485 (CCVT_0489) - 518791..519354 (-) 564 WP_018136894.1 hypothetical protein -
  CCVT_RS02490 (CCVT_0490) gatC 519473..519760 (+) 288 WP_018136893.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CCVT_RS02495 (CCVT_0491) pilT 519764..520885 (+) 1122 WP_011991975.1 type IV pilus twitching motility protein PilT Machinery gene
  CCVT_RS02500 (CCVT_0492) - 520887..521504 (+) 618 WP_018136892.1 CvpA family protein -
  CCVT_RS02505 (CCVT_0493) - 521501..521968 (+) 468 WP_009650275.1 Fur family transcriptional regulator -
  CCVT_RS02510 (CCVT_0494) lysS 521973..523481 (+) 1509 WP_026175513.1 lysine--tRNA ligase -
  CCVT_RS02515 (CCVT_0495) - 523478..524722 (+) 1245 WP_018136890.1 serine hydroxymethyltransferase -
  CCVT_RS02520 (CCVT_0496) - 524722..525264 (+) 543 WP_009650298.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41363.45 Da        Isoelectric Point: 7.2082

>NTDB_id=447941 CCVT_RS02495 WP_011991975.1 519764..520885(+) (pilT) [Campylobacter curvus strain ATCC 35224]
MDLIQTNNNIVQAPPRPTNTDIQTLLKTVVFNKASDLHLVARSEPQIRIDGTLRPLELGILSGKDIETLCYALITDAQKS
ELENNKELDFAIELPSIGRFRGNYYYTMNGDLAAAFRIIPIEIPSLDELKSPQIFKDIIKREKGLILVTGPTGSGKSTTL
AAMLNEINLNYRKHIITIEDPVEFVHSNKKALFSHRNIGTDTHSYARALKFSLREDPDIILVGEMRDKETISIAITAAET
GHLVFGTLHTNSAIQTINRIVDSFDGSEQLQVRNMLSVSLTAVISQSLLPKTNGGRCAIHEILINNMAVANLIRENKVHQ
IYSQMQLNQQQTGMCTQTQSLIKAIRGGLITKENALRYSTSQQELLNTMGAGA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=447941 CCVT_RS02495 WP_011991975.1 519764..520885(+) (pilT) [Campylobacter curvus strain ATCC 35224]
ATGGATCTGATACAAACGAACAATAACATAGTCCAAGCACCGCCAAGACCGACAAATACAGACATACAAACATTACTAAA
AACCGTTGTTTTCAATAAAGCCAGTGACCTGCATCTTGTCGCAAGATCCGAGCCTCAAATAAGGATCGACGGTACATTGC
GTCCTCTCGAGCTTGGAATTTTAAGCGGCAAAGACATAGAGACTCTGTGTTATGCACTGATAACGGACGCTCAAAAAAGC
GAGCTTGAAAACAATAAAGAGCTTGACTTTGCGATAGAGCTACCAAGTATCGGACGCTTTCGCGGTAACTACTACTACAC
TATGAATGGCGATCTGGCGGCCGCATTTCGTATCATACCTATCGAAATTCCATCTCTTGACGAGCTGAAATCTCCTCAGA
TTTTTAAAGACATCATAAAGCGCGAAAAGGGGCTTATTTTAGTCACCGGACCTACCGGTAGCGGTAAATCGACTACCCTT
GCAGCTATGCTAAACGAGATAAATTTAAACTATCGAAAGCATATCATAACGATAGAAGACCCGGTAGAATTCGTCCATAG
CAACAAAAAAGCGCTATTTTCACACAGAAATATCGGCACTGATACGCACTCATACGCTAGAGCGCTTAAATTTTCACTGC
GCGAAGACCCCGACATCATCCTGGTGGGCGAGATGAGAGATAAAGAGACGATATCTATCGCGATAACAGCGGCTGAAACC
GGACACCTAGTTTTTGGAACTCTACATACGAATTCCGCTATCCAGACGATAAACAGGATAGTCGACAGCTTTGACGGCAG
CGAGCAGCTTCAGGTGCGAAACATGCTCAGCGTCTCGCTAACGGCGGTCATCTCGCAAAGCCTGCTTCCAAAGACCAACG
GCGGCAGGTGTGCGATACATGAAATTTTGATCAACAACATGGCCGTTGCAAATTTGATCCGTGAAAACAAGGTGCATCAG
ATATACTCTCAGATGCAGCTAAATCAGCAGCAAACTGGCATGTGCACGCAGACGCAGTCTTTGATAAAGGCCATAAGAGG
CGGCCTTATAACCAAAGAAAATGCGTTGCGTTATTCGACCAGTCAGCAAGAGCTTTTAAATACTATGGGTGCAGGTGCTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GX44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.754

92.493

0.488
  pilT Neisseria meningitidis 8013

52.464

92.493

0.485
  pilT Acinetobacter nosocomialis M2

52.616

92.225

0.485
  pilT Acinetobacter baumannii D1279779

52.616

92.225

0.485
  pilT Acinetobacter baumannii strain A118

52.616

92.225

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

88.472

0.48
  pilT Vibrio cholerae strain A1552

54.242

88.472

0.48
  pilT Legionella pneumophila strain Lp02

51.94

89.812

0.466
  pilT Legionella pneumophila strain ERS1305867

51.94

89.812

0.466
  pilT Acinetobacter baylyi ADP1

52.424

88.472

0.464
  pilT Pseudomonas aeruginosa PAK

50.746

89.812

0.456
  pilT Pseudomonas stutzeri DSM 10701

49.558

90.885

0.45
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.011

96.247

0.424