Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH213_RS15935 Genome accession   NZ_CP051684
Coordinates   3495755..3496891 (-) Length   378 a.a.
NCBI ID   WP_169112860.1    Uniprot ID   -
Organism   Duganella dendranthematis strain AF9R3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3490755..3501891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH213_RS15920 (HH213_15920) glcF 3491390..3492613 (+) 1224 WP_169112858.1 glycolate oxidase subunit GlcF -
  HH213_RS15925 (HH213_15925) - 3492605..3495103 (-) 2499 WP_229263023.1 bifunctional diguanylate cyclase/phosphodiesterase -
  HH213_RS15930 (HH213_15930) - 3495270..3495755 (-) 486 WP_169112859.1 glutathione peroxidase -
  HH213_RS15935 (HH213_15935) pilU 3495755..3496891 (-) 1137 WP_169112860.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH213_RS15940 (HH213_15940) pilT 3496904..3497947 (-) 1044 WP_169112861.1 type IV pilus twitching motility protein PilT Machinery gene
  HH213_RS15945 (HH213_15945) - 3498008..3498721 (+) 714 WP_169115217.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH213_RS15950 (HH213_15950) proC 3498725..3499546 (+) 822 WP_169112862.1 pyrroline-5-carboxylate reductase -
  HH213_RS15955 (HH213_15955) - 3499652..3499987 (-) 336 WP_110847340.1 YqjD family protein -
  HH213_RS15960 (HH213_15960) ubiA 3500083..3500937 (-) 855 WP_169112863.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41797.33 Da        Isoelectric Point: 6.4357

>NTDB_id=440438 HH213_RS15935 WP_169112860.1 3495755..3496891(-) (pilU) [Duganella dendranthematis strain AF9R3]
MERDQASKFMFDLLRLMLAKKGSDLFITAGFPPAVKIDGKMTPVSAQPLTAAHTLDLARSIMNDKQAAGFELTKEANFAI
SPGDIGRFRVSAFVQMGAVGMVLRTINSDIPKLEELGLPEVLKDVVMSKRGLVIMVGATGSGKSTTLAAMVGYRNEHSYG
HIITIEDPVEFVHPHKNCIVTQREVGVDTDDWAVALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPKKESKGRAVAIEIMLNSPLISDLIFKGEVHEIKELMKKSRELG
MQTFDQSLFDLHEADLITYEDALRNADSVNDLRLTIKLEGKAAKNRDLSAGTEHLGLI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=440438 HH213_RS15935 WP_169112860.1 3495755..3496891(-) (pilU) [Duganella dendranthematis strain AF9R3]
ATGGAACGCGATCAGGCCTCAAAATTCATGTTCGACTTGCTGCGCCTGATGTTGGCCAAGAAGGGCTCGGACCTGTTCAT
CACCGCCGGCTTCCCGCCGGCCGTCAAGATCGACGGCAAGATGACACCGGTGTCGGCGCAGCCGCTCACCGCCGCCCACA
CGCTCGATCTGGCCCGTTCCATCATGAACGACAAGCAGGCCGCCGGCTTCGAGCTGACCAAGGAAGCCAACTTCGCCATC
AGCCCCGGCGACATCGGCCGCTTCCGCGTCTCCGCCTTCGTGCAGATGGGCGCGGTCGGGATGGTGCTGCGGACCATTAA
CTCCGACATCCCCAAGCTGGAGGAACTCGGCCTGCCCGAAGTGCTGAAGGATGTGGTGATGTCCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAGTCGACCACCTTGGCCGCCATGGTCGGCTACCGCAATGAACACAGCTACGGC
CACATCATCACCATCGAAGATCCGGTCGAGTTCGTTCATCCGCACAAGAACTGCATCGTCACCCAGCGCGAAGTTGGTGT
CGATACCGACGACTGGGCGGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAAATCCGCG
ACCGCGAGACCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCTTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAGCAGCTGTTGATGGATTTGTCGCTGAACCT
GAAAGGCATGATTTCGCAACGCCTGATTCCAAAGAAGGAATCGAAGGGCCGCGCGGTGGCCATCGAAATCATGCTGAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGAGGTGCACGAGATCAAGGAGCTGATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTGCACGAAGCCGACCTGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCCGTCAACGACCTGCGCCTGACCATCAAGCTGGAAGGCAAGGCCGCAAAGAACCGCGACCTGTCGGCCGGCACTGAAC
ATTTGGGACTCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.466

96.561

0.603
  pilU Acinetobacter baylyi ADP1

59.104

94.444

0.558
  pilU Vibrio cholerae strain A1552

58.651

90.212

0.529
  pilT Pseudomonas aeruginosa PAK

45.593

87.037

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.161

87.037

0.376
  pilT Acinetobacter baylyi ADP1

44.062

84.656

0.373
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365