NTDB

Overview

Name	pilE	Type	Machinery gene
Locus tag	HBA47_RS09490	Genome accession	NZ_CP050136
Coordinates	1807562..1808032 (-)	Length	156 a.a.
NCBI ID	WP_154700047.1	Uniprot ID	-
Organism	Kingella kingae strain ATCC 23332
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1802562..1813032

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBA47_RS09455 (HBA47_09495)	-	1803500..1803658 (+)	159	WP_019390092.1	hypothetical protein	-
HBA47_RS09460 (HBA47_09500)	-	1803661..1804386 (+)	726	WP_019390093.1	metal ABC transporter ATP-binding protein	-
HBA47_RS09465 (HBA47_09505)	-	1804435..1804827 (+)	393	WP_003791724.1	pyrimidine dimer DNA glycosylase/endonuclease V	-
HBA47_RS09470 (HBA47_09510)	-	1804865..1805755 (+)	891	WP_019390094.1	metal ABC transporter permease	-
HBA47_RS09480 (HBA47_09520)	-	1806201..1806977 (-)	777	WP_019390095.1	fimb protein	-
HBA47_RS09485 (HBA47_09525)	-	1806989..1807417 (-)	429	WP_154700048.1	pilin	-
HBA47_RS09490 (HBA47_09530)	pilE	1807562..1808032 (-)	471	WP_154700047.1	pilin	Machinery gene
HBA47_RS09495 (HBA47_09535)	recJ	1808389..1810101 (+)	1713	WP_154700046.1	single-stranded-DNA-specific exonuclease RecJ	-
HBA47_RS09500 (HBA47_09540)	-	1810213..1810947 (+)	735	WP_003791735.1	histidine phosphatase family protein	-
HBA47_RS09505 (HBA47_09545)	-	1810986..1811531 (+)	546	WP_154700045.1	gamma carbonic anhydrase family protein	-
HBA47_RS09510 (HBA47_09550)	-	1811522..1812571 (+)	1050	WP_019390102.1	metallophosphoesterase	-

Sequence

Protein

Download Length: 156 a.a. Molecular weight: 16042.17 Da Isoelectric Point: 8.9857

                                >NTDB_id=430302 HBA47_RS09490 WP_154700047.1 1807562..1808032(-) (pilE) [Kingella kingae strain ATCC 23332]

MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTKRSRVSEGLSLAGGVKSALTEYYASNNAWPANNAAAGIADKGQIT

GNAVSSVEVSATGSGAGGTGQITVTFNDKVKDKATLIMKGVGNDAGSVKWTCTDGTLDTKYRPSECRGTTGANTNG

Nucleotide

Download Length: 471 bp

                                >NTDB_id=430302 HBA47_RS09490 WP_154700047.1 1807562..1808032(-) (pilE) [Kingella kingae strain ATCC 23332]

ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATTGTAATCGCTATTATCGGTATTTTGGCTGCGATTGC

ATTGCCAGCTTACCAAGATTACACTAAACGTTCACGCGTATCTGAAGGTTTGAGCTTGGCTGGTGGCGTGAAAAGTGCTT

TGACTGAGTACTACGCTTCTAACAATGCATGGCCTGCTAATAATGCAGCAGCTGGTATTGCAGATAAAGGCCAAATTACA

GGTAATGCAGTATCAAGCGTGGAAGTATCAGCTACTGGTTCTGGTGCTGGAGGTACAGGTCAAATTACTGTTACTTTCAA

CGATAAAGTAAAAGACAAGGCTACTTTGATTATGAAAGGTGTAGGCAACGATGCTGGTTCAGTTAAATGGACTTGTACTG

ATGGTACCTTGGATACTAAATACCGTCCTTCTGAATGCCGTGGCACTACTGGGGCTAATACTAATGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria gonorrhoeae strain FA1090	47.368	100	0.519
pilE	Neisseria gonorrhoeae MS11	47.561	100	0.5
pilA2	Legionella pneumophila strain ERS1305867	52.448	91.667	0.481
comP	Acinetobacter baylyi ADP1	48.052	98.718	0.474
pilA2	Legionella pneumophila str. Paris	51.748	91.667	0.474
pilA	Ralstonia pseudosolanacearum GMI1000	40.116	100	0.442
pilA/pilA1	Eikenella corrodens VA1	39.103	100	0.391
pilA	Vibrio cholerae strain A1552	39.216	98.077	0.385
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	39.216	98.077	0.385
pilA	Vibrio cholerae C6706	39.216	98.077	0.385
pilA/pilAI	Pseudomonas stutzeri DSM 10701	38.816	97.436	0.378
pilA	Acinetobacter baumannii strain A118	37.662	98.718	0.372
pilA	Haemophilus influenzae 86-028NP	38.255	95.513	0.365

Detailed information NTG0430302