Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   HBA50_RS00865 Genome accession   NZ_CP050133
Coordinates   167131..168072 (+) Length   313 a.a.
NCBI ID   WP_045500284.1    Uniprot ID   -
Organism   Streptococcus cristatus ATCC 51100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162131..173072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA50_RS00835 (HBA50_00835) - 162328..163563 (+) 1236 WP_045500270.1 oligosaccharide flippase family protein -
  HBA50_RS00840 (HBA50_00840) wecB 163560..164648 (+) 1089 WP_045500273.1 non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase -
  HBA50_RS00845 (HBA50_00845) comW 164801..165028 (+) 228 WP_045500276.1 sigma(X)-activator ComW -
  HBA50_RS00855 (HBA50_00855) - 165271..166539 (+) 1269 WP_045500279.1 CapA family protein -
  HBA50_RS00860 (HBA50_00860) - 166674..167045 (+) 372 WP_045500282.1 DUF1033 family protein -
  HBA50_RS00865 (HBA50_00865) comYA 167131..168072 (+) 942 WP_045500284.1 competence type IV pilus ATPase ComGA Machinery gene
  HBA50_RS00870 (HBA50_00870) comYB 168020..169036 (+) 1017 WP_045500287.1 competence type IV pilus assembly protein ComGB Machinery gene
  HBA50_RS00875 (HBA50_00875) comYC 169033..169350 (+) 318 WP_045500288.1 competence type IV pilus major pilin ComGC Machinery gene
  HBA50_RS00880 (HBA50_00880) comYD 169310..169744 (+) 435 WP_045500291.1 competence type IV pilus minor pilin ComGD Machinery gene
  HBA50_RS00885 (HBA50_00885) comGE 169710..170003 (+) 294 WP_200893262.1 competence type IV pilus minor pilin ComGE -
  HBA50_RS00890 (HBA50_00890) comGF/cglF 169987..170424 (+) 438 WP_045500297.1 competence type IV pilus minor pilin ComGF Machinery gene
  HBA50_RS00895 (HBA50_00895) comGG 170405..170803 (+) 399 WP_045500299.1 competence type IV pilus minor pilin ComGG -
  HBA50_RS00900 (HBA50_00900) comYH 170884..171849 (+) 966 WP_045500301.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35842.87 Da        Isoelectric Point: 5.4415

>NTDB_id=430185 HBA50_RS00865 WP_045500284.1 167131..168072(+) (comYA) [Streptococcus cristatus ATCC 51100]
MVQKIAKEMIQLARQEAAQDIYLIPKSSCYELYMRVGDERRFIQTYDFDLLSSVISHFKFVSGMNVGEKRRSQLGSCDYD
CGEATVSLRLSTVGDYRGYESLVIRLLHDEERELRFWFDNLPDLREKVNSRGLYLFSGPVGSGKTTLMHQLAQMRFGQQQ
VMSIEDPVEIKQENMLQLQLNETIGMTYDSLIKLSLRHRPDLLIIGEIRDQETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEDELRMVLQGVCYQRLIGGGGVIDFVNQDYQQHEASLWNQQIDQLFADGHITADQRQAEKIIYA

Nucleotide


Download         Length: 942 bp        

>NTDB_id=430185 HBA50_RS00865 WP_045500284.1 167131..168072(+) (comYA) [Streptococcus cristatus ATCC 51100]
ATGGTTCAAAAAATTGCAAAGGAAATGATTCAATTGGCCAGACAGGAAGCGGCCCAAGATATCTATCTGATTCCCAAGAG
CTCTTGCTATGAGCTCTATATGCGGGTGGGAGATGAACGGCGTTTTATTCAAACCTATGATTTTGATTTACTGTCGTCAG
TCATCAGCCATTTTAAATTTGTCTCGGGGATGAATGTCGGGGAGAAGAGGCGCAGTCAGCTAGGCTCCTGCGATTATGAT
TGTGGAGAGGCCACGGTTTCGCTCCGTCTGTCGACTGTCGGGGATTATCGTGGCTACGAGAGTCTGGTGATTCGGCTCTT
ACACGATGAGGAGCGTGAGCTGCGTTTTTGGTTTGATAATCTGCCTGATCTTCGTGAAAAAGTAAATTCTCGGGGCCTTT
ATCTGTTTTCAGGGCCGGTTGGCAGTGGAAAGACGACGCTGATGCACCAGCTGGCTCAGATGAGATTTGGCCAACAACAG
GTCATGTCTATCGAAGACCCTGTGGAAATCAAGCAGGAAAACATGCTCCAGCTTCAGCTGAATGAAACCATCGGGATGAC
CTACGACAGCCTTATCAAATTGTCTCTGCGCCACCGACCGGACCTTTTGATTATAGGGGAAATCCGCGACCAAGAAACGG
CTCGTGCGGTGGTAAGGGCTAGTTTGACAGGGGCCACGGTCTTTTCAACCATCCATGCCAAGAGCATTCGCGGAGTGTAT
GAGCGCCTGTTAGAGCTGGGTGTCAGTGAGGACGAGCTGCGCATGGTGTTACAGGGCGTCTGTTACCAGCGCTTAATCGG
GGGAGGAGGTGTGATCGACTTTGTCAACCAAGACTATCAACAGCATGAAGCCAGTCTCTGGAACCAGCAGATTGACCAGC
TTTTTGCAGATGGACATATCACAGCTGATCAAAGGCAGGCGGAAAAAATTATCTACGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

74.121

100

0.741
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

73.163

100

0.732
  comGA/cglA/cilD Streptococcus pneumoniae D39

73.163

100

0.732
  comGA/cglA/cilD Streptococcus pneumoniae R6

73.163

100

0.732
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

73.163

100

0.732
  comYA Streptococcus mutans UA159

67.524

99.361

0.671
  comYA Streptococcus mutans UA140

67.524

99.361

0.671
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

64.516

99.042

0.639
  comGA Lactococcus lactis subsp. cremoris KW2

55.769

99.681

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

40.794

88.498

0.361