Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   HBA50_RS00890 Genome accession   NZ_CP050133
Coordinates   169987..170424 (+) Length   145 a.a.
NCBI ID   WP_045500297.1    Uniprot ID   -
Organism   Streptococcus cristatus ATCC 51100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 164987..175424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA50_RS00855 (HBA50_00855) - 165271..166539 (+) 1269 WP_045500279.1 CapA family protein -
  HBA50_RS00860 (HBA50_00860) - 166674..167045 (+) 372 WP_045500282.1 DUF1033 family protein -
  HBA50_RS00865 (HBA50_00865) comYA 167131..168072 (+) 942 WP_045500284.1 competence type IV pilus ATPase ComGA Machinery gene
  HBA50_RS00870 (HBA50_00870) comYB 168020..169036 (+) 1017 WP_045500287.1 competence type IV pilus assembly protein ComGB Machinery gene
  HBA50_RS00875 (HBA50_00875) comYC 169033..169350 (+) 318 WP_045500288.1 competence type IV pilus major pilin ComGC Machinery gene
  HBA50_RS00880 (HBA50_00880) comYD 169310..169744 (+) 435 WP_045500291.1 competence type IV pilus minor pilin ComGD Machinery gene
  HBA50_RS00885 (HBA50_00885) comGE 169710..170003 (+) 294 WP_200893262.1 competence type IV pilus minor pilin ComGE -
  HBA50_RS00890 (HBA50_00890) comGF/cglF 169987..170424 (+) 438 WP_045500297.1 competence type IV pilus minor pilin ComGF Machinery gene
  HBA50_RS00895 (HBA50_00895) comGG 170405..170803 (+) 399 WP_045500299.1 competence type IV pilus minor pilin ComGG -
  HBA50_RS00900 (HBA50_00900) comYH 170884..171849 (+) 966 WP_045500301.1 class I SAM-dependent methyltransferase Machinery gene
  HBA50_RS00905 (HBA50_00905) - 171885..173078 (+) 1194 WP_045500304.1 acetate kinase -
  HBA50_RS00910 (HBA50_00910) - 173389..174279 (-) 891 WP_045500311.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16973.47 Da        Isoelectric Point: 9.6216

>NTDB_id=430189 HBA50_RS00890 WP_045500297.1 169987..170424(+) (comGF/cglF) [Streptococcus cristatus ATCC 51100]
MLKNVKIKAFTLLETLVALLVLSGGMLVFQSMTKLLAFELRHQQENKQQEWLLFVDQLETELSRSQFDRVENDKIYVKQD
GKNIALGKSRSDDFRKTDSSGRGYQPMIYGLKAAPVSQEGDLVRFRFRFENNLEREYIYRVQDKS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=430189 HBA50_RS00890 WP_045500297.1 169987..170424(+) (comGF/cglF) [Streptococcus cristatus ATCC 51100]
GTGTTAAAAAACGTTAAAATTAAAGCTTTTACACTCTTAGAGACTCTAGTCGCCCTGCTGGTCTTGAGCGGTGGTATGCT
AGTCTTTCAGTCGATGACCAAGCTTCTGGCTTTTGAACTGCGACATCAACAGGAAAATAAGCAGCAGGAATGGCTCTTAT
TTGTCGATCAGTTGGAGACGGAGCTATCGCGCAGCCAATTTGACCGAGTCGAAAACGACAAAATCTATGTCAAACAGGAT
GGTAAAAATATAGCTTTGGGCAAGTCTCGGAGTGATGATTTCCGCAAGACGGATTCTTCGGGGCGTGGCTATCAACCCAT
GATCTATGGACTCAAAGCTGCTCCGGTCAGTCAGGAGGGGGACCTTGTTCGCTTTCGCTTCCGGTTTGAAAATAATTTAG
AAAGAGAGTACATCTATCGTGTTCAAGATAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

67.143

96.552

0.648
  comGF/cglF Streptococcus mitis SK321

63.571

96.552

0.614
  comGF/cglF Streptococcus pneumoniae D39

62.857

96.552

0.607
  comGF/cglF Streptococcus pneumoniae TIGR4

62.857

96.552

0.607
  comGF/cglF Streptococcus pneumoniae R6

62.857

96.552

0.607
  comGF/cglF Streptococcus pneumoniae Rx1

62.857

96.552

0.607
  comYF Streptococcus mutans UA140

41.379

100

0.414
  comYF Streptococcus mutans UA159

40.69

100

0.407
  comGF Lactococcus lactis subsp. cremoris KW2

41.727

95.862

0.4