Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MICA_RS07250 Genome accession   NC_016026
Coordinates   1525716..1526906 (-) Length   396 a.a.
NCBI ID   WP_014103077.1    Uniprot ID   G2KPN5
Organism   Micavibrio aeruginosavorus ARL-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1520716..1531906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MICA_RS07230 (MICA_1532) - 1520874..1521638 (-) 765 WP_014103073.1 hypothetical protein -
  MICA_RS07235 (MICA_1533) - 1521652..1522422 (-) 771 WP_014103074.1 hypothetical protein -
  MICA_RS07240 (MICA_1534) - 1522530..1524410 (-) 1881 WP_014103075.1 type II and III secretion system family protein -
  MICA_RS07245 (MICA_1535) - 1524589..1525719 (-) 1131 WP_236619873.1 PilT/PilU family type 4a pilus ATPase -
  MICA_RS07250 (MICA_1536) pilT 1525716..1526906 (-) 1191 WP_014103077.1 type IV pilus twitching motility protein PilT Machinery gene
  MICA_RS07255 (MICA_1537) - 1527036..1527860 (-) 825 WP_014103078.1 A24 family peptidase -
  MICA_RS07260 (MICA_1538) - 1527882..1529471 (-) 1590 WP_014103079.1 tetratricopeptide repeat protein -
  MICA_RS07265 (MICA_1539) - 1529622..1530380 (-) 759 WP_041793919.1 ABC transporter ATP-binding protein -
  MICA_RS07270 (MICA_1540) - 1530410..1531102 (-) 693 WP_014103081.1 hypothetical protein -
  MICA_RS07275 (MICA_1541) - 1531099..1531743 (-) 645 WP_014103082.1 hypothetical protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43403.10 Da        Isoelectric Point: 5.2195

>NTDB_id=42513 MICA_RS07250 WP_014103077.1 1525716..1526906(-) (pilT) [Micavibrio aeruginosavorus ARL-13]
MDILQLLRFTIQNNASDLHLSPTNPPVIRVNGEMKRIKADPLQSDDIRTMLYSVMNEDQRADFERELELDFAIAYGEKAR
FRVNAFTTRNGTAAVFRSIPSLIPTMEELELPPIIRRFAELEKGLVLVTGPTGSGKSTTLASIINYINENHASHIITVED
PVEFFHASKKSVVNHREIGTDTKSFSKALRSSLREDPDVILVGEMRDYETISLALTAAETGHLVFGTLHSNTAAKTIDRI
IDVFPSGDKEMVRAMLASSLQGVVAQSLLKRADGSGRVGAFEILVGTNAVRNLIRENQIAQLYSMIQTGSRYGMTTLEDS
VNALLEQGVIDKDEARRALLKTTEDEGARDEDSVAASLGGGKMSETLTRKQAAVGRALGAQPRKSGEDDSGEGYTF

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=42513 MICA_RS07250 WP_014103077.1 1525716..1526906(-) (pilT) [Micavibrio aeruginosavorus ARL-13]
TTGGATATTCTTCAGCTTTTGCGCTTTACGATTCAGAATAATGCGTCTGACTTGCACTTGAGTCCGACCAACCCACCGGT
CATTCGGGTGAACGGGGAAATGAAGCGGATCAAGGCCGATCCGTTGCAAAGCGATGATATTCGCACCATGCTTTATTCCG
TGATGAATGAAGACCAGCGCGCCGATTTCGAGCGGGAGCTGGAGCTGGATTTTGCCATCGCCTACGGTGAAAAGGCCCGT
TTCCGTGTGAACGCCTTTACCACACGAAACGGCACAGCCGCCGTTTTCCGTTCCATTCCGTCCCTGATCCCGACGATGGA
AGAGCTGGAATTGCCACCGATTATCCGCCGCTTTGCCGAGCTGGAGAAGGGGCTGGTTCTGGTGACCGGTCCGACGGGTT
CGGGTAAATCGACCACGCTGGCTTCGATCATCAATTACATTAACGAAAATCACGCCAGCCACATTATTACGGTCGAAGAC
CCGGTTGAATTTTTCCACGCGTCGAAAAAATCGGTGGTGAACCACCGTGAAATCGGCACGGACACCAAGTCCTTCTCTAA
AGCCCTGCGCAGCTCGTTGCGTGAAGACCCGGATGTCATCCTGGTGGGGGAGATGCGGGACTATGAAACGATTTCGCTGG
CGTTGACCGCGGCGGAAACCGGGCACTTGGTGTTTGGAACCCTGCACTCGAACACGGCGGCCAAAACCATCGACCGTATC
ATTGACGTGTTCCCGTCGGGGGACAAGGAAATGGTTCGGGCCATGCTGGCCAGTTCGTTGCAGGGCGTTGTGGCCCAGTC
GCTGTTGAAGCGTGCGGATGGGTCGGGCCGGGTTGGGGCGTTTGAGATCCTGGTGGGGACCAACGCCGTGCGAAACCTGA
TCCGTGAAAACCAGATTGCCCAGCTTTATTCCATGATCCAGACCGGGTCGCGTTACGGGATGACCACGCTGGAAGACTCC
GTCAATGCCTTGTTGGAACAGGGTGTGATTGATAAGGACGAGGCGCGCCGCGCCTTGCTGAAAACCACGGAAGATGAGGG
CGCGCGCGACGAAGACAGCGTTGCGGCCTCGCTGGGTGGTGGCAAGATGTCCGAAACCCTGACCCGGAAACAGGCGGCCG
TTGGTCGCGCCCTGGGTGCGCAGCCGCGCAAATCTGGCGAGGATGATTCAGGCGAGGGCTACACATTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2KPN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

59.467

85.354

0.508
  pilT Acinetobacter baumannii D1279779

59.467

85.354

0.508
  pilT Acinetobacter baumannii strain A118

59.467

85.354

0.508
  pilT Acinetobacter baylyi ADP1

57.849

86.869

0.503
  pilT Legionella pneumophila strain ERS1305867

58.529

85.859

0.503
  pilT Legionella pneumophila strain Lp02

58.529

85.859

0.503
  pilT Neisseria gonorrhoeae MS11

58.457

85.101

0.497
  pilT Neisseria meningitidis 8013

58.457

85.101

0.497
  pilT Pseudomonas aeruginosa PAK

57.143

84.848

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.845

84.848

0.482
  pilT Vibrio cholerae strain A1552

56.845

84.848

0.482
  pilT Pseudomonas stutzeri DSM 10701

56.845

84.848

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.863

92.172

0.46

Multiple sequence alignment