Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SPPN_RS10360 Genome accession   NC_015875
Coordinates   1984088..1985029 (-) Length   313 a.a.
NCBI ID   WP_000249547.1    Uniprot ID   -
Organism   Streptococcus pseudopneumoniae IS7493     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1979088..1990029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPPN_RS10320 (SPPN_10445) - 1979345..1979821 (+) 477 WP_000777684.1 GNAT family N-acetyltransferase -
  SPPN_RS10325 (SPPN_10450) - 1980018..1980860 (+) 843 WP_000840058.1 helix-turn-helix domain-containing protein -
  SPPN_RS10330 (SPPN_10455) comGG/cglG 1981322..1981735 (-) 414 WP_000265635.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPPN_RS10335 (SPPN_10460) comGF/cglF 1981713..1982174 (-) 462 WP_000250511.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPPN_RS10340 (SPPN_10465) comGE/cglE 1982137..1982439 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPPN_RS10345 (SPPN_10470) comGD/cglD 1982402..1982836 (-) 435 WP_044012294.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPPN_RS10350 (SPPN_10475) comGC/cglC 1982799..1983122 (-) 324 WP_000738647.1 comG operon protein ComGC Machinery gene
  SPPN_RS10355 (SPPN_10480) comGB/cglB 1983124..1984140 (-) 1017 WP_080564700.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPPN_RS10360 (SPPN_10485) comGA/cglA/cilD 1984088..1985029 (-) 942 WP_000249547.1 competence type IV pilus ATPase ComGA Machinery gene
  SPPN_RS10365 (SPPN_10490) - 1985105..1985470 (-) 366 WP_000286407.1 DUF1033 family protein -
  SPPN_RS10370 (SPPN_10495) - 1985621..1986658 (-) 1038 Protein_2082 zinc-dependent alcohol dehydrogenase family protein -
  SPPN_RS10375 (SPPN_10500) - 1986977..1987216 (-) 240 WP_000907298.1 hypothetical protein -
  SPPN_RS10380 (SPPN_10505) - 1987330..1987764 (-) 435 WP_000355912.1 cytidine deaminase family protein -
  SPPN_RS10385 (SPPN_10510) gltX 1987778..1989238 (-) 1461 WP_000031058.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35607.57 Da        Isoelectric Point: 6.0086

>NTDB_id=42037 SPPN_RS10360 WP_000249547.1 1984088..1985029(-) (comGA/cglA/cilD) [Streptococcus pseudopneumoniae IS7493]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYNFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=42037 SPPN_RS10360 WP_000249547.1 1984088..1985029(-) (comGA/cglA/cilD) [Streptococcus pseudopneumoniae IS7493]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCCCGGAAAAAAGGAGCGCAAGATATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGCTGTTATAACTTCGAAAAATTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGTCAACTCGGTTCCTGTGACTATGAA
TATGACCAGAAGATGGCGTCCCTACGTTTATCTACCGTAGGCGATTATCGAGGGCATGAGAGTTTAGTCATTCGCTTGTT
GCACGATGAAGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCCTGATGCATGAATTGTCCAAGTCACTCTTTAAGGGGCAACAA
GTTATGTCCATCGAAGATCCTGTAGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCAACAGTTTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.083

100

0.981
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.083

100

0.981
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.083

100

0.981
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.083

100

0.981
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.769

99.681

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

41.544

86.901

0.361

Multiple sequence alignment