Detailed information NTG0419637

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EBO27_RS02055
• Genome accession: NZ_CP061034
• Coordinates: 442741..443889 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 437741..448889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBO27_RS02030 (EBO27_02030)	-	438892..439188 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EBO27_RS02035 (EBO27_02035)	-	439185..439778 (-)	594	WP_003084544.1	YggT family protein	-
EBO27_RS02040 (EBO27_02040)	proC	439789..440610 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
EBO27_RS02045 (EBO27_02045)	-	440622..441314 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
EBO27_RS02050 (EBO27_02050)	pilT	441529..442563 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EBO27_RS02055 (EBO27_02055)	pilU	442741..443889 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EBO27_RS02060 (EBO27_02060)	czcD	443897..444796 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EBO27_RS02065 (EBO27_02065)	-	444950..445354 (+)	405	WP_003084560.1	NINE protein	-
EBO27_RS02070 (EBO27_02070)	-	445597..446970 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
EBO27_RS02075 (EBO27_02075)	-	446967..448151 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=419637 EBO27_RS02055 WP_003100959.1 442741..443889(+) (pilU) [Pseudomonas aeruginosa strain PA3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=419637 EBO27_RS02055 WP_003100959.1 442741..443889(+) (pilU) [Pseudomonas aeruginosa strain PA3]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382