Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GUY17_RS19575 Genome accession   NZ_CP048031
Coordinates   4562202..4563908 (+) Length   568 a.a.
NCBI ID   WP_162024080.1    Uniprot ID   A0AAE6SVT1
Organism   Shewanella sp. Arc9-LZ     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4557202..4568908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUY17_RS19560 (GUY17_19590) nadC 4557784..4558662 (+) 879 WP_162024078.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GUY17_RS21310 (GUY17_19595) pilA/pilAI 4559732..4560166 (+) 435 WP_302476619.1 pilin Machinery gene
  GUY17_RS19570 (GUY17_19600) - 4560637..4562004 (-) 1368 WP_162024079.1 O-antigen ligase -
  GUY17_RS19575 (GUY17_19605) pilB 4562202..4563908 (+) 1707 WP_162024080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GUY17_RS19580 (GUY17_19610) pilC 4564018..4565277 (+) 1260 WP_162024081.1 type II secretion system F family protein Machinery gene
  GUY17_RS19585 (GUY17_19615) pilD 4565309..4566235 (+) 927 WP_162024082.1 A24 family peptidase Machinery gene
  GUY17_RS19590 (GUY17_19620) coaE 4566232..4566861 (+) 630 WP_162024083.1 dephospho-CoA kinase -
  GUY17_RS19595 (GUY17_19625) zapD 4566854..4567588 (+) 735 WP_162024084.1 cell division protein ZapD -
  GUY17_RS19600 (GUY17_19630) yacG 4567631..4567843 (+) 213 WP_162024085.1 DNA gyrase inhibitor YacG -
  GUY17_RS19605 (GUY17_19635) mutT 4567867..4568262 (+) 396 WP_162024086.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62787.24 Da        Isoelectric Point: 5.4707

>NTDB_id=419632 GUY17_RS19575 WP_162024080.1 4562202..4563908(+) (pilB) [Shewanella sp. Arc9-LZ]
MASTSLNLGLSSLFVKKNLLSDQVITDAISESRKSKKTLVSSLISMKALSAREIAELCYEEYGTPLLDLNEFDITQISEE
LLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSLGLHAEAILVEDDKLNKALEKILEEDISGLGLEGIDEEA
LANIEVTDTSRDDEPSVDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSNRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPACKHEETIPVAEMQRLGFS
EANIAKGFSVFKPVGCELCSGGYKGRVGIYEVMKMSDEIARIIMEGGNSLQIATLAKQQGMRDLRQSGLLKVIHGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=419632 GUY17_RS19575 WP_162024080.1 4562202..4563908(+) (pilB) [Shewanella sp. Arc9-LZ]
ATGGCGTCAACAAGTCTTAACTTAGGGCTCTCAAGCTTGTTCGTTAAAAAGAATCTACTTAGCGATCAAGTGATTACTGA
TGCCATATCTGAATCACGAAAAAGTAAGAAGACTTTAGTTAGTAGCCTAATCTCTATGAAGGCACTATCAGCTCGAGAAA
TAGCTGAATTATGCTATGAAGAATATGGCACTCCATTACTCGATTTAAATGAATTTGATATTACTCAAATATCAGAAGAG
CTTTTAAATAAAAAACTTATCGAAAAACATAAGTGCCTGCCATTATTTAAACGCGGTAACCGTCTCTACATCGCTACATC
CGACCCTACCAATATTGCAGCTCTTGAAGACTTCCAGTTTAGTTTAGGTTTACATGCCGAAGCTATTCTTGTAGAGGACG
ATAAGCTCAATAAAGCACTTGAAAAAATTCTTGAAGAAGACATTAGTGGTCTTGGATTAGAAGGTATTGATGAAGAAGCC
CTTGCTAATATTGAAGTAACTGATACAAGCCGAGACGATGAACCTTCAGTAGATAGCAGTGATGATGCACCAATTGTTAT
TTATATTAATAAAATTCTTACCGATGCCATACGTAAAGGTGCCTCAGATTTACATTTTGAACCTTACGAAAAACGCTACC
GTATTCGTTTTCGTATCGACGGTATCTTGCATGAAGTATCCGAGCCGCCGGTCAATCTATCCAACAGGATTTCCGCCCGT
TTAAAAGTCATGTCAAAACTCGATATTGCTGAGCGTCGAGTGCCGCAAGATGGCCGTATAAAAATGAAACTGTCGCGAAC
CAAATCTATCGATTTTCGTGTCAGTACCTTGCCCACCATCTGGGGCGAGAAGATCGTAATGCGTATATTGGATTCATCTT
CTGCGCAGCTTGGTATTGAAAAGCTCGGCTATGAAGATGATCAACGTTTGTTATATGAAGAGATGCTCGCTAAACCCCAA
GGAATGATTTTAGTTACCGGCCCTACTGGTTCGGGTAAAACAGTCTCCTTGTATACCGGCCTCAATATTCTTAATACCGA
AGAGCGTAATATTTCCACCGCCGAAGATCCGGTCGAAATTAACCTCGAAGGGGTTAATCAGGTCCATATTAATTTAAAAG
CAGGCTTAACTTTCGCATCTGCGCTGCGCTCATTCTTACGTCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
TTAGAAACCGCGGAAATAGCTATAAAAGCGGCGCAAACAGGCCATTTAGTGTTATCAACCCTACATACTAATTCTGCCGC
GGAAACCTTAACCCGTTTAATCAATATGGGTGTACCTGGTTATAACATTGCCAGCTCAGTCAATTTAATTATTGCCCAAC
GACTTGCCAGACGCTTATGCCCAGCATGTAAACACGAAGAGACGATTCCAGTTGCTGAAATGCAACGACTAGGATTTAGT
GAAGCCAATATAGCCAAAGGCTTCAGCGTATTCAAACCTGTCGGTTGCGAGCTATGCTCCGGTGGTTATAAGGGCCGTGT
CGGTATTTATGAAGTGATGAAAATGAGCGATGAAATCGCCCGTATTATTATGGAAGGTGGTAACTCACTCCAAATTGCTA
CCTTAGCAAAGCAGCAAGGCATGCGCGATTTACGTCAATCTGGGCTACTTAAAGTCATTCACGGTGTAACCAGTATTGCC
GAAATTAACCGTGTCACCAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.363

98.944

0.577
  pilB Acinetobacter baumannii D1279779

58.289

98.768

0.576
  pilB Legionella pneumophila strain ERS1305867

56.228

98.944

0.556
  pilB Vibrio cholerae strain A1552

54.083

97.007

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

51.504

99.472

0.512
  pilB Vibrio campbellii strain DS40M4

50.444

99.12

0.5
  pilF Neisseria gonorrhoeae MS11

46.903

99.472

0.467
  pilF Thermus thermophilus HB27

38.036

98.592

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

93.838

0.371

Multiple sequence alignment