Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GSR16_RS18510 Genome accession   NZ_CP047241
Coordinates   3993556..3994008 (-) Length   150 a.a.
NCBI ID   WP_159879948.1    Uniprot ID   -
Organism   Aquitalea denitrificans strain 5YN1-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3988556..3999008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSR16_RS18495 rpoH 3988801..3989664 (+) 864 WP_159879942.1 RNA polymerase sigma factor RpoH -
  GSR16_RS18500 - 3989799..3991586 (-) 1788 WP_159879944.1 PglL family O-oligosaccharyltransferase -
  GSR16_RS18505 - 3991681..3993495 (-) 1815 WP_159879946.1 PglL family O-oligosaccharyltransferase -
  GSR16_RS18510 pilA 3993556..3994008 (-) 453 WP_159879948.1 pilin Machinery gene
  GSR16_RS21385 - 3994025..3994519 (-) 495 WP_276608560.1 pilin -
  GSR16_RS18520 - 3994636..3995985 (+) 1350 WP_159879950.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  GSR16_RS18525 - 3996151..3997650 (+) 1500 WP_159879952.1 hypothetical protein -
  GSR16_RS18530 proB 3997729..3998847 (+) 1119 WP_159879954.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15430.65 Da        Isoelectric Point: 7.8118

>NTDB_id=411803 GSR16_RS18510 WP_159879948.1 3993556..3994008(-) (pilA) [Aquitalea denitrificans strain 5YN1-3]
MQKQIQQGFTLIELMIVVAIIGILAAIAIPAYQDYTVRAKVSEGLAAVAAAKTAVSEAAITYGTMPGSQASAGFTSVATK
YVSSVTYTQTDSTHGVLTVMMNAGNVGGNVTTSTNQFSLTGLLASGTVTWTCAQAASQPIATQYLPANCR

Nucleotide


Download         Length: 453 bp        

>NTDB_id=411803 GSR16_RS18510 WP_159879948.1 3993556..3994008(-) (pilA) [Aquitalea denitrificans strain 5YN1-3]
ATGCAAAAACAAATCCAGCAGGGTTTTACCCTGATCGAACTGATGATTGTTGTGGCAATCATTGGTATTTTGGCTGCCAT
CGCCATTCCGGCGTATCAGGACTACACCGTACGCGCCAAGGTATCTGAAGGTTTGGCTGCTGTTGCTGCCGCCAAAACAG
CGGTTTCCGAGGCTGCCATTACCTACGGCACCATGCCGGGTAGTCAGGCTTCTGCGGGCTTTACCTCTGTAGCAACAAAG
TATGTTTCCTCAGTTACTTATACCCAGACTGATAGCACTCATGGCGTACTGACGGTAATGATGAATGCTGGCAATGTGGG
TGGTAACGTTACCACGAGTACAAATCAATTCTCTCTAACTGGTCTCTTGGCTAGTGGTACTGTTACTTGGACATGTGCTC
AGGCTGCATCGCAGCCTATAGCGACTCAATATCTCCCGGCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)

Pilin (bacterial filament) (Pilin)

(37-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.061

100

0.507
  pilA2 Legionella pneumophila str. Paris

50

97.333

0.487
  pilA2 Legionella pneumophila strain ERS1305867

50

97.333

0.487
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.473
  comP Acinetobacter baylyi ADP1

45.455

100

0.467
  pilA/pilA1 Eikenella corrodens VA1

44.304

100

0.467
  pilE Neisseria gonorrhoeae strain FA1090

40.741

100

0.44
  pilE Neisseria gonorrhoeae MS11

39.264

100

0.427
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.854

100

0.407
  pilA Vibrio campbellii strain DS40M4

37.255

100

0.38
  pilA Glaesserella parasuis strain SC1401

36.774

100

0.38
  pilA Haemophilus influenzae 86-028NP

41.304

92

0.38
  pilA Acinetobacter baumannii strain A118

38.095

98

0.373

Multiple sequence alignment