Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY10_RS12405 Genome accession   NZ_CP046767
Coordinates   2638907..2639329 (-) Length   140 a.a.
NCBI ID   WP_158125403.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2013V-1300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2633907..2644329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY10_RS12385 coaE 2634418..2635026 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  GPY10_RS12390 pilD 2635029..2635898 (-) 870 WP_024374403.1 prepilin peptidase Machinery gene
  GPY10_RS12395 pilC 2635950..2637176 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  GPY10_RS12400 pilB 2637212..2638900 (-) 1689 WP_158151729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY10_RS12405 pilA 2638907..2639329 (-) 423 WP_158125403.1 pilin Machinery gene
  GPY10_RS12410 nadC 2639559..2640446 (-) 888 WP_158129993.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY10_RS12415 ampD 2640598..2641149 (+) 552 WP_158151730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY10_RS12420 fldB 2641154..2641675 (-) 522 WP_020331982.1 flavodoxin FldB -
  GPY10_RS12425 xerD 2641816..2642724 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  GPY10_RS12430 dsbC 2642741..2643505 (+) 765 WP_158151731.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14585.77 Da        Isoelectric Point: 9.4843

>NTDB_id=407431 GPY10_RS12405 WP_158125403.1 2638907..2639329(-) (pilA) [Vibrio fluvialis strain 2013V-1300]
MNANQRKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEASAALASITGQRVNVESYVITEGVFPTTTQVPVPSSA
LGTITYANAASAAGDIIFTFKSSGVSPDVVSKKVELKRTSAGAWSCSTTVATDLKPKGCS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=407431 GPY10_RS12405 WP_158125403.1 2638907..2639329(-) (pilA) [Vibrio fluvialis strain 2013V-1300]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTAGCAATTATTGGGGTGTT
GGCAGCAATCGCTGTTCCTCAGTATCAAAAGTATGTTGCAAAAAGTGAGGCTTCAGCAGCACTAGCATCTATAACAGGAC
AGCGAGTTAATGTTGAGTCTTATGTAATAACAGAAGGCGTATTTCCTACAACAACCCAGGTACCCGTACCTTCCTCTGCA
TTGGGAACAATAACCTATGCTAATGCAGCATCTGCTGCTGGGGATATCATATTTACATTCAAATCTTCAGGTGTAAGCCC
AGATGTTGTCAGTAAAAAAGTGGAACTAAAGAGAACAAGTGCTGGTGCATGGAGCTGTAGTACAACTGTAGCGACAGACT
TGAAGCCTAAAGGCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.669

100

0.536
  pilA Vibrio cholerae strain A1552

49.669

100

0.536
  pilA Vibrio cholerae C6706

49.669

100

0.536
  pilA Pseudomonas aeruginosa PAK

43.919

100

0.464
  pilA Acinetobacter baumannii strain A118

43.151

100

0.45
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

100

0.443
  pilA Vibrio parahaemolyticus RIMD 2210633

39.041

100

0.407
  comP Acinetobacter baylyi ADP1

34.81

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.333

100

0.379
  pilA2 Legionella pneumophila str. Paris

34.667

100

0.371
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.806

95.714

0.371
  pilA Acinetobacter nosocomialis M2

40.8

89.286

0.364

Multiple sequence alignment