Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPW74_RS07360 Genome accession   NZ_CP046737
Coordinates   1662886..1663350 (+) Length   154 a.a.
NCBI ID   WP_114741846.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1657886..1668350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS07335 dsbC 1658444..1659196 (-) 753 WP_123012493.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GPW74_RS07340 xerD 1659213..1660121 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GPW74_RS07345 fldB 1660259..1660777 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPW74_RS07350 ampD 1660908..1661453 (-) 546 WP_069503756.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW74_RS07355 nadC 1661751..1662641 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW74_RS07360 pilA 1662886..1663350 (+) 465 WP_114741846.1 pilin Machinery gene
  GPW74_RS07365 pilB 1663351..1665039 (+) 1689 WP_114731868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW74_RS07370 pilC 1665082..1666308 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPW74_RS07375 pilD 1666366..1667241 (+) 876 WP_088128389.1 A24 family peptidase Machinery gene
  GPW74_RS07380 coaE 1667238..1667846 (+) 609 WP_069503760.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15915.00 Da        Isoelectric Point: 8.9666

>NTDB_id=406858 GPW74_RS07360 WP_114741846.1 1662886..1663350(+) (pilA) [Vibrio cholerae strain 2015V-1126]
MNAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALATLTGLKTNAEAYTVETGSFPTDAQKAQLGTP
SSAMGTIAYANTSSSGAAGTITFTFSGTVSPDLKPSGTASEIRLSRDGSGTWTCTSKSTLVEGVRPKGCTNTYN

Nucleotide


Download         Length: 465 bp        

>NTDB_id=406858 GPW74_RS07360 WP_114741846.1 1662886..1663350(+) (pilA) [Vibrio cholerae strain 2015V-1126]
ATGAACGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCTGTAATTGGGGTGCT
AGCAGCAATTGCGGTACCTCAATACCAGAAATACGTAGCGAAAAGTGAAGCAGCTTCTGCATTAGCTACGTTGACTGGCT
TAAAAACCAATGCAGAGGCTTATACCGTTGAAACTGGTTCTTTCCCAACTGATGCACAGAAAGCTCAATTAGGAACACCA
TCATCTGCAATGGGTACTATTGCATATGCTAATACCAGTAGCAGTGGTGCTGCTGGAACAATTACATTTACCTTTAGTGG
TACAGTTAGCCCAGACTTAAAACCTAGTGGAACAGCTAGCGAAATCAGACTTTCTAGAGACGGTAGCGGAACTTGGACTT
GCACATCAAAATCTACGTTAGTTGAAGGTGTTCGCCCTAAAGGCTGTACAAATACATATAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

52.229

100

0.532
  pilA Vibrio cholerae strain A1552

52.229

100

0.532
  pilA Vibrio cholerae C6706

52.229

100

0.532
  pilA Pseudomonas aeruginosa PAK

46.154

100

0.468
  pilA Vibrio parahaemolyticus RIMD 2210633

43.537

95.455

0.416
  pilA Acinetobacter baumannii strain A118

40.397

98.052

0.396
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.838

96.104

0.364
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.584

96.753

0.364

Multiple sequence alignment