Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPW74_RS07365 Genome accession   NZ_CP046737
Coordinates   1663351..1665039 (+) Length   562 a.a.
NCBI ID   WP_114731868.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1658351..1670039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS07335 dsbC 1658444..1659196 (-) 753 WP_123012493.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GPW74_RS07340 xerD 1659213..1660121 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GPW74_RS07345 fldB 1660259..1660777 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPW74_RS07350 ampD 1660908..1661453 (-) 546 WP_069503756.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW74_RS07355 nadC 1661751..1662641 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW74_RS07360 pilA 1662886..1663350 (+) 465 WP_114741846.1 pilin Machinery gene
  GPW74_RS07365 pilB 1663351..1665039 (+) 1689 WP_114731868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW74_RS07370 pilC 1665082..1666308 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPW74_RS07375 pilD 1666366..1667241 (+) 876 WP_088128389.1 A24 family peptidase Machinery gene
  GPW74_RS07380 coaE 1667238..1667846 (+) 609 WP_069503760.1 dephospho-CoA kinase -
  GPW74_RS07385 zapD 1667878..1668618 (+) 741 WP_000207194.1 cell division protein ZapD -
  GPW74_RS07390 yacG 1668776..1668973 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62071.18 Da        Isoelectric Point: 6.0863

>NTDB_id=406859 GPW74_RS07365 WP_114731868.1 1663351..1665039(+) (pilB) [Vibrio cholerae strain 2015V-1126]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDESPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=406859 GPW74_RS07365 WP_114731868.1 1663351..1665039(+) (pilB) [Vibrio cholerae strain 2015V-1126]
ATGCTAACTAACCTTGTTGCTATCTTACGTCAGGCTGAATTAATCAGCGCGACGCAAGAGCAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGAACTTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAGGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTTGGGCTACGTGAA
CTGATTACCCGCTACGATGCCTTACCGATTGCCAAGCAAGGCAACTTATTGCTGCTCGCGGTCTCTGACCCGACTTTGCT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACTGGGCTGCAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTCAAGGTCAGCGATGACGAGCTGCAATCCATTGAAGATCTCAGCCAAGATGAGTCCCCCGTCAGTCGCTTTATCAA
CCAAGTGTTACTCGATGCGGTGCGTAAAGGCGCCTCTGACATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCGACACTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTTGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTACAACCCGCAGCAAAAGCAACTCTACCTCAATGCCCTCAAAAGGCCGCAAGGGATGATT
TTAATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGACCCAGTAGAAATAAACCTCTCTGGGATTAACCAAGTGCAAGTGCAACCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTACGCCAAGATCCGGATGTGGTGATGGTCGGTGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAATGCACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGTATTCATCAAATTGAACGTTTAGCTAAAGCCAATG
GTATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGTTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

52.642

90.925

0.479
  pilB Acinetobacter baylyi ADP1

50.577

92.527

0.468
  pilB Acinetobacter baumannii D1279779

50.385

92.527

0.466
  pilF Neisseria gonorrhoeae MS11

45.552

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

37.086

100

0.399
  pilF Thermus thermophilus HB27

39.556

96.263

0.381
  pilB Haemophilus influenzae 86-028NP

39.279

93.772

0.368

Multiple sequence alignment