Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPW74_RS07370 Genome accession   NZ_CP046737
Coordinates   1665082..1666308 (+) Length   408 a.a.
NCBI ID   WP_000648506.1    Uniprot ID   A0A0E3W4T9
Organism   Vibrio cholerae strain 2015V-1126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660082..1671308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS07345 fldB 1660259..1660777 (+) 519 WP_000690112.1 flavodoxin FldB -
  GPW74_RS07350 ampD 1660908..1661453 (-) 546 WP_069503756.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW74_RS07355 nadC 1661751..1662641 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW74_RS07360 pilA 1662886..1663350 (+) 465 WP_114741846.1 pilin Machinery gene
  GPW74_RS07365 pilB 1663351..1665039 (+) 1689 WP_114731868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW74_RS07370 pilC 1665082..1666308 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPW74_RS07375 pilD 1666366..1667241 (+) 876 WP_088128389.1 A24 family peptidase Machinery gene
  GPW74_RS07380 coaE 1667238..1667846 (+) 609 WP_069503760.1 dephospho-CoA kinase -
  GPW74_RS07385 zapD 1667878..1668618 (+) 741 WP_000207194.1 cell division protein ZapD -
  GPW74_RS07390 yacG 1668776..1668973 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW74_RS07395 parC 1669022..1671307 (-) 2286 WP_000102513.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45090.38 Da        Isoelectric Point: 10.3424

>NTDB_id=406860 GPW74_RS07370 WP_000648506.1 1665082..1666308(+) (pilC) [Vibrio cholerae strain 2015V-1126]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=406860 GPW74_RS07370 WP_000648506.1 1665082..1666308(+) (pilC) [Vibrio cholerae strain 2015V-1126]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTGAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGTGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGCAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGCTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCAGAACTGCCTTGGTTTACGC
AGCAAGTACTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCTATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGACGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCAGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3W4T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

74

98.039

0.725
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment