Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GPW74_RS07375 Genome accession   NZ_CP046737
Coordinates   1666366..1667241 (+) Length   291 a.a.
NCBI ID   WP_088128389.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661366..1672241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS07355 nadC 1661751..1662641 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW74_RS07360 pilA 1662886..1663350 (+) 465 WP_114741846.1 pilin Machinery gene
  GPW74_RS07365 pilB 1663351..1665039 (+) 1689 WP_114731868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW74_RS07370 pilC 1665082..1666308 (+) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  GPW74_RS07375 pilD 1666366..1667241 (+) 876 WP_088128389.1 A24 family peptidase Machinery gene
  GPW74_RS07380 coaE 1667238..1667846 (+) 609 WP_069503760.1 dephospho-CoA kinase -
  GPW74_RS07385 zapD 1667878..1668618 (+) 741 WP_000207194.1 cell division protein ZapD -
  GPW74_RS07390 yacG 1668776..1668973 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW74_RS07395 parC 1669022..1671307 (-) 2286 WP_000102513.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32490.79 Da        Isoelectric Point: 7.6295

>NTDB_id=406861 GPW74_RS07375 WP_088128389.1 1666366..1667241(+) (pilD) [Vibrio cholerae strain 2015V-1126]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTSKEGMGYGDFKLLAALGAWLGWQQLPIIVLLSSVVGV
IFGLIQLRQQKKGINMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWLGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=406861 GPW74_RS07375 WP_088128389.1 1666366..1667241(+) (pilD) [Vibrio cholerae strain 2015V-1126]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACACCGATTCGCGTGATT
GATAATATCCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTAATTGCCACTCACTTTCCGTTCGGGGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACCAGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTGGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTATCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCAATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTACTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGTTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

98.625

100

0.986
  pilD Vibrio campbellii strain DS40M4

75.347

98.969

0.746
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment