Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   GO995_RS01020 Genome accession   NZ_CP046624
Coordinates   164176..165117 (+) Length   313 a.a.
NCBI ID   WP_157628641.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_G3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 159176..170117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO995_RS01010 (GO995_01010) rpoC 159944..163579 (+) 3636 WP_074564834.1 DNA-directed RNA polymerase subunit beta' -
  GO995_RS01015 (GO995_01015) - 163735..164103 (+) 369 WP_157628640.1 DUF1033 family protein -
  GO995_RS01020 (GO995_01020) comYA 164176..165117 (+) 942 WP_157628641.1 competence type IV pilus ATPase ComGA Machinery gene
  GO995_RS01025 (GO995_01025) comYB 165041..166084 (+) 1044 WP_202913541.1 competence type IV pilus assembly protein ComGB Machinery gene
  GO995_RS01030 (GO995_01030) comYC 166084..166386 (+) 303 WP_157628643.1 competence type IV pilus major pilin ComGC Machinery gene
  GO995_RS01035 (GO995_01035) comYD 166361..166792 (+) 432 WP_157628644.1 competence type IV pilus minor pilin ComGD Machinery gene
  GO995_RS01040 (GO995_01040) comYE 166746..167039 (+) 294 WP_157628645.1 competence type IV pilus minor pilin ComGE Machinery gene
  GO995_RS01045 (GO995_01045) comYF 167023..167460 (+) 438 WP_157628646.1 competence type IV pilus minor pilin ComGF Machinery gene
  GO995_RS01050 (GO995_01050) comGG 167432..167782 (+) 351 WP_133018088.1 competence type IV pilus minor pilin ComGG -
  GO995_RS01055 (GO995_01055) comYH 167836..168792 (+) 957 WP_006531743.1 class I SAM-dependent methyltransferase Machinery gene
  GO995_RS01060 (GO995_01060) - 168846..170045 (+) 1200 WP_039697418.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35667.92 Da        Isoelectric Point: 6.2647

>NTDB_id=406139 GO995_RS01020 WP_157628641.1 164176..165117(+) (comYA) [Streptococcus ruminicola strain CNU_G3]
MIQEKAKQLIKDAVEKNAQDIYIVPKKERYEIYQRVGDERQFIQELASDEMISVISHFKFVAGMNVGEKRRSQLGSCDYE
FAEGEEISLRLSTVGDYRGRESLVIRLLYSGRHDLQYWFDGMKNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPGK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSRQELENSLRAIIYQRLIGGGGVVDFAKGDFEHYPPTRWNEQIKSLARDGHISLEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=406139 GO995_RS01020 WP_157628641.1 164176..165117(+) (comYA) [Streptococcus ruminicola strain CNU_G3]
ATGATTCAGGAAAAAGCAAAGCAGCTGATTAAGGATGCTGTTGAAAAGAATGCGCAAGATATTTACATTGTTCCTAAGAA
GGAGCGCTATGAAATTTATCAACGTGTTGGTGATGAACGTCAGTTTATTCAGGAGCTTGCTAGTGATGAGATGATAAGTG
TGATTAGTCACTTTAAATTTGTAGCTGGGATGAATGTTGGTGAGAAACGTCGTAGTCAGCTTGGTTCTTGTGACTATGAA
TTTGCTGAGGGTGAAGAGATTTCACTGCGTTTATCCACTGTTGGAGATTATCGAGGGCGTGAGAGCCTGGTCATCAGACT
TCTTTATTCAGGTCGTCATGATTTGCAATATTGGTTCGATGGGATGAAGAATATTTTAGAGGCGATTGATAGTCGAGGTC
TTTATCTTTTTTCCGGGCCGGTAGGGAGTGGGAAAACCACTCTCATGTACCAATTAGTTCGTGAAAAATTTCCTGGAAAG
CAGGTGATTACGATTGAGGACCCTGTTGAGATAAAACAAGACAATATGTTGCAATTGCAGTTGAATGATGAAATCGGGAT
GACTTATGATAATCTCATTAAACTCTCGCTTCGCCATAGACCTGATATTTTAATTATTGGAGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTAACAGGTGTGCTCGTCTTTTCAACCATCCATGCGAAAAGTATTCCGGGTGTT
TATGCTAGGTTACTGGAGTTGGGAGTTAGTAGACAAGAGCTTGAAAATAGTTTACGAGCGATTATTTATCAACGATTAAT
TGGCGGTGGAGGTGTTGTGGATTTTGCCAAAGGAGATTTTGAACACTATCCCCCAACAAGGTGGAATGAGCAAATTAAAA
GCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

71.061

99.361

0.706
  comYA Streptococcus mutans UA140

70.74

99.361

0.703
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.097

99.042

0.665
  comYA Streptococcus gordonii str. Challis substr. CH1

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.385

99.681

0.652
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.423

99.681

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.423

99.681

0.642
  comGA Lactococcus lactis subsp. cremoris KW2

54.313

100

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

43.066

87.54

0.377

Multiple sequence alignment