Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GO995_RS01025 Genome accession   NZ_CP046624
Coordinates   165041..166084 (+) Length   347 a.a.
NCBI ID   WP_202913541.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_G3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160041..171084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO995_RS01015 (GO995_01015) - 163735..164103 (+) 369 WP_157628640.1 DUF1033 family protein -
  GO995_RS01020 (GO995_01020) comYA 164176..165117 (+) 942 WP_157628641.1 competence type IV pilus ATPase ComGA Machinery gene
  GO995_RS01025 (GO995_01025) comYB 165041..166084 (+) 1044 WP_202913541.1 competence type IV pilus assembly protein ComGB Machinery gene
  GO995_RS01030 (GO995_01030) comYC 166084..166386 (+) 303 WP_157628643.1 competence type IV pilus major pilin ComGC Machinery gene
  GO995_RS01035 (GO995_01035) comYD 166361..166792 (+) 432 WP_157628644.1 competence type IV pilus minor pilin ComGD Machinery gene
  GO995_RS01040 (GO995_01040) comYE 166746..167039 (+) 294 WP_157628645.1 competence type IV pilus minor pilin ComGE Machinery gene
  GO995_RS01045 (GO995_01045) comYF 167023..167460 (+) 438 WP_157628646.1 competence type IV pilus minor pilin ComGF Machinery gene
  GO995_RS01050 (GO995_01050) comGG 167432..167782 (+) 351 WP_133018088.1 competence type IV pilus minor pilin ComGG -
  GO995_RS01055 (GO995_01055) comYH 167836..168792 (+) 957 WP_006531743.1 class I SAM-dependent methyltransferase Machinery gene
  GO995_RS01060 (GO995_01060) - 168846..170045 (+) 1200 WP_039697418.1 acetate kinase -
  GO995_RS01065 (GO995_01065) - 170204..170401 (+) 198 WP_074561137.1 helix-turn-helix transcriptional regulator -
  GO995_RS01070 (GO995_01070) - 170417..170617 (+) 201 WP_157628647.1 hypothetical protein -
  GO995_RS01075 (GO995_01075) - 170618..171073 (+) 456 WP_238385877.1 ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39622.99 Da        Isoelectric Point: 9.6038

>NTDB_id=406140 GO995_RS01025 WP_202913541.1 165041..166084(+) (comYB) [Streptococcus ruminicola strain CNU_G3]
MSKLKALLGTDILALRKQKSKKLPFKKQQKVIQLFNNLFNSGFNLTEIVSFLRRSQLLAEVYVDSMQESLLSGASLADMM
AKLGFSDTIVTQIALADVHGNCQQSLLKIDGYLASMSVVRKKLIEVATYPLILFSFLILIMLGLKNYLLPQLENQNFATQ
IISHFPMIFLGGFALLALGILLGVLYAKRLSPIYLYSQLSRLPIFGRYVRLYLTAYYAREWGNLIGQGIELMEIVELMQR
QKSRLFQEIGKDMQEALLSGQSFHQKVLDYPFFLRELSLMIEYGEVKSKLGRELDIYAEETWQNFFSQLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=406140 GO995_RS01025 WP_202913541.1 165041..166084(+) (comYB) [Streptococcus ruminicola strain CNU_G3]
ATGAGCAAATTAAAAGCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAAAAA
ACAGCAGAAGGTGATTCAATTATTTAACAATCTCTTTAACAGTGGCTTTAATTTAACAGAAATTGTTTCTTTTTTGAGAC
GAAGTCAATTGTTGGCAGAAGTTTATGTGGATAGTATGCAAGAATCTTTGCTAAGCGGGGCAAGTTTGGCAGATATGATG
GCTAAATTAGGCTTTTCAGATACAATTGTCACTCAGATTGCTTTGGCAGATGTTCATGGAAATTGTCAGCAGAGTTTGTT
AAAAATTGATGGCTACTTGGCCAGTATGTCAGTTGTTCGAAAGAAATTGATTGAAGTGGCGACTTATCCTTTGATTTTGT
TCAGCTTTTTAATTCTGATTATGTTGGGCTTGAAAAATTATTTGCTACCGCAGTTGGAAAATCAAAATTTTGCGACTCAG
ATTATCAGCCATTTTCCTATGATTTTTTTAGGTGGCTTTGCTCTTTTAGCTTTAGGAATTCTGCTTGGGGTTCTGTATGC
CAAGCGCTTATCGCCAATTTATCTGTATAGTCAGTTGAGTCGTTTGCCAATCTTTGGGCGGTACGTGCGTCTTTATTTGA
CCGCCTATTATGCGCGTGAGTGGGGAAATCTTATCGGGCAAGGTATTGAGCTTATGGAGATTGTAGAGCTCATGCAGCGG
CAAAAATCACGCCTTTTTCAGGAAATTGGAAAAGACATGCAGGAAGCTTTATTGTCAGGTCAATCTTTTCATCAGAAAGT
GCTGGATTACCCTTTCTTTTTGCGTGAACTTAGCTTGATGATTGAGTACGGTGAAGTCAAATCAAAATTAGGGCGTGAAC
TGGATATTTACGCAGAGGAAACTTGGCAGAATTTCTTTAGTCAATTGACACAGGCAACGCAACTGATTCAGCCTTTGGTC
TTTGTCTTTGTTGCTTTAATCATCGTTTTAATTTATGTGGCAATGCTTTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

65.19

91.066

0.594
  comYB Streptococcus mutans UA159

65.19

91.066

0.594
  comYB Streptococcus gordonii str. Challis substr. CH1

58.235

97.983

0.571
  comGB/cglB Streptococcus mitis NCTC 12261

58.16

97.118

0.565
  comGB/cglB Streptococcus mitis SK321

57.27

97.118

0.556
  comGB/cglB Streptococcus pneumoniae Rx1

56.677

97.118

0.55
  comGB/cglB Streptococcus pneumoniae D39

56.677

97.118

0.55
  comGB/cglB Streptococcus pneumoniae R6

56.677

97.118

0.55
  comGB/cglB Streptococcus pneumoniae TIGR4

56.677

97.118

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

51.488

96.83

0.499

Multiple sequence alignment