Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   GO995_RS01055 Genome accession   NZ_CP046624
Coordinates   167836..168792 (+) Length   318 a.a.
NCBI ID   WP_006531743.1    Uniprot ID   A0A380KLA4
Organism   Streptococcus ruminicola strain CNU_G3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162836..173792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO995_RS01015 (GO995_01015) - 163735..164103 (+) 369 WP_157628640.1 DUF1033 family protein -
  GO995_RS01020 (GO995_01020) comYA 164176..165117 (+) 942 WP_157628641.1 competence type IV pilus ATPase ComGA Machinery gene
  GO995_RS01025 (GO995_01025) comYB 165041..166084 (+) 1044 WP_202913541.1 competence type IV pilus assembly protein ComGB Machinery gene
  GO995_RS01030 (GO995_01030) comYC 166084..166386 (+) 303 WP_157628643.1 competence type IV pilus major pilin ComGC Machinery gene
  GO995_RS01035 (GO995_01035) comYD 166361..166792 (+) 432 WP_157628644.1 competence type IV pilus minor pilin ComGD Machinery gene
  GO995_RS01040 (GO995_01040) comYE 166746..167039 (+) 294 WP_157628645.1 competence type IV pilus minor pilin ComGE Machinery gene
  GO995_RS01045 (GO995_01045) comYF 167023..167460 (+) 438 WP_157628646.1 competence type IV pilus minor pilin ComGF Machinery gene
  GO995_RS01050 (GO995_01050) comGG 167432..167782 (+) 351 WP_133018088.1 competence type IV pilus minor pilin ComGG -
  GO995_RS01055 (GO995_01055) comYH 167836..168792 (+) 957 WP_006531743.1 class I SAM-dependent methyltransferase Machinery gene
  GO995_RS01060 (GO995_01060) - 168846..170045 (+) 1200 WP_039697418.1 acetate kinase -
  GO995_RS01065 (GO995_01065) - 170204..170401 (+) 198 WP_074561137.1 helix-turn-helix transcriptional regulator -
  GO995_RS01070 (GO995_01070) - 170417..170617 (+) 201 WP_157628647.1 hypothetical protein -
  GO995_RS01075 (GO995_01075) - 170618..171073 (+) 456 WP_238385877.1 ABC transporter permease -
  GO995_RS01080 (GO995_01080) - 171089..171724 (+) 636 WP_157628648.1 CPBP family intramembrane glutamic endopeptidase -
  GO995_RS01085 (GO995_01085) proC 171759..172529 (-) 771 WP_039697503.1 pyrroline-5-carboxylate reductase -
  GO995_RS01090 (GO995_01090) pepA 172591..173658 (-) 1068 WP_074603339.1 glutamyl aminopeptidase -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 35885.95 Da        Isoelectric Point: 4.4551

>NTDB_id=406145 GO995_RS01055 WP_006531743.1 167836..168792(+) (comYH) [Streptococcus ruminicola strain CNU_G3]
MNFEKIETAYGLILENIQLIENELKTHIYDALIEQNSFYLGAEGANETVAANNEKLRQLDLTKEEWRRAFQFIFIKAAQT
EALQANHQFTPDAIGFILMFLIENLTASKELDVLEIGSGTGNLAQTLLNNSSKDLNYLGIEVDDLLIDLSASIAEVMDSK
AQFIQEDAVRPQILKESDVIISDLPVGFYPNDEIAKRYKVASSDEHTYAHHLLMEQSLKYLKKDGIAVFLAPVSLLTSKQ
SDLLKAWLKDYADVIAVITLPESIFGNAANAKSIFVLKKQAEHTPETFVYPLADLQSREALTDFIEKFKKWNVENMIF

Nucleotide


Download         Length: 957 bp        

>NTDB_id=406145 GO995_RS01055 WP_006531743.1 167836..168792(+) (comYH) [Streptococcus ruminicola strain CNU_G3]
ATGAATTTTGAAAAAATCGAAACAGCTTATGGGCTGATTCTAGAAAATATACAGTTAATCGAAAATGAGTTGAAAACACA
CATTTACGATGCACTTATTGAACAAAACTCTTTTTATCTTGGTGCTGAAGGTGCCAATGAGACAGTAGCTGCAAATAATG
AAAAACTACGCCAACTTGATTTAACTAAAGAAGAATGGCGTCGTGCTTTTCAGTTTATTTTCATTAAAGCTGCTCAAACA
GAAGCACTTCAGGCAAACCACCAATTTACACCTGATGCTATTGGCTTTATCTTAATGTTCCTTATTGAGAATTTGACAGC
CTCGAAAGAACTTGATGTTTTAGAAATTGGTAGCGGAACAGGTAACCTTGCACAAACCTTGCTCAATAATTCATCTAAAG
ACTTGAATTACCTTGGAATTGAAGTTGATGATTTGTTGATTGACTTGTCAGCAAGTATTGCCGAAGTGATGGATTCTAAA
GCTCAGTTCATTCAAGAAGATGCTGTGCGCCCACAGATTCTTAAGGAAAGTGATGTCATCATCAGTGACCTTCCAGTCGG
CTTCTATCCAAATGATGAGATTGCAAAACGCTACAAAGTAGCAAGTAGTGATGAACACACTTATGCGCATCATTTGTTGA
TGGAACAATCTCTCAAATATCTCAAAAAAGATGGTATTGCTGTCTTTTTAGCACCGGTTAGTCTTTTGACAAGTAAACAA
AGTGATTTGTTAAAGGCATGGTTGAAGGATTACGCTGATGTTATTGCGGTGATTACTTTACCAGAATCTATTTTCGGCAA
TGCGGCCAATGCTAAGTCAATTTTTGTCTTGAAGAAACAGGCTGAACATACTCCAGAGACATTTGTTTATCCGCTTGCTG
ACTTGCAAAGTCGCGAAGCTTTAACAGATTTTATTGAGAAATTTAAAAAATGGAATGTTGAAAATATGATTTTTTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(69-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380KLA4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA140

68.038

99.371

0.676
  comYH Streptococcus mutans UA159

67.722

99.371

0.673

Multiple sequence alignment