Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   IXO35_RS15450 Genome accession   NZ_CP046488
Coordinates   3325968..3326393 (-) Length   141 a.a.
NCBI ID   WP_011408068.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain IXO35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3313843..3328007 3325968..3326393 within 0


Gene organization within MGE regions


Location: 3313843..3328007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXO35_RS15395 (IXO35_015500) sucD 3313843..3314718 (-) 876 WP_011258368.1 succinate--CoA ligase subunit alpha -
  IXO35_RS15400 (IXO35_015505) sucC 3314743..3315912 (-) 1170 WP_011258367.1 ADP-forming succinate--CoA ligase subunit beta -
  IXO35_RS15405 (IXO35_015510) - 3316146..3317759 (+) 1614 WP_011258366.1 HAMP domain-containing sensor histidine kinase -
  IXO35_RS15410 (IXO35_015515) pilR 3318030..3319481 (+) 1452 WP_171970756.1 sigma-54 dependent transcriptional regulator Regulator
  IXO35_RS15415 (IXO35_015520) - 3319554..3319760 (+) 207 WP_027703281.1 YhfG family protein -
  IXO35_RS15420 - 3319822..3319983 (+) 162 WP_153296778.1 hypothetical protein -
  IXO35_RS15425 - 3320071..3320331 (+) 261 WP_011408073.1 Fic family protein -
  IXO35_RS15430 (IXO35_015530) pilB 3320340..3322073 (-) 1734 WP_011408072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IXO35_RS15435 (IXO35_015535) - 3322108..3323634 (-) 1527 WP_075241021.1 hypothetical protein -
  IXO35_RS15440 (IXO35_015540) - 3323604..3324233 (-) 630 WP_011408070.1 hypothetical protein -
  IXO35_RS15445 (IXO35_015545) - 3324244..3325833 (-) 1590 WP_011408069.1 phosphoethanolamine transferase -
  IXO35_RS15450 (IXO35_015550) pilA2 3325968..3326393 (-) 426 WP_011408068.1 pilin Machinery gene
  IXO35_RS15455 (IXO35_015555) pilC 3326748..3328007 (+) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14442.76 Da        Isoelectric Point: 9.0090

>NTDB_id=404691 IXO35_RS15450 WP_011408068.1 3325968..3326393(-) (pilA2) [Xanthomonas oryzae pv. oryzae strain IXO35]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVANVTLANSGYSFPGATK
YVSNVTIADGTGLVTVTSTVPNAAGTILLTPKDVGGGQLKWTCSSAILTKYLPAECRSSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=404691 IXO35_RS15450 WP_011408068.1 3325968..3326393(-) (pilA2) [Xanthomonas oryzae pv. oryzae strain IXO35]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAACTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACTCATTCCCGGGTGCCACGAAG
TATGTCAGTAACGTCACCATCGCCGATGGTACGGGCCTAGTCACTGTAACTTCTACCGTGCCGAACGCAGCCGGTACCAT
CCTGCTGACCCCGAAGGATGTCGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCCTCACCAAGTACCTGCCGG
CGGAGTGCCGTAGCTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.471

96.454

0.496
  pilA2 Legionella pneumophila strain ERS1305867

51.471

96.454

0.496
  pilA Ralstonia pseudosolanacearum GMI1000

41.818

100

0.489
  comP Acinetobacter baylyi ADP1

43.537

100

0.454
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

37.278

100

0.447
  pilE Neisseria gonorrhoeae MS11

37.5

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.446

98.582

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA Acinetobacter baumannii strain A118

38.298

100

0.383
  pilA Pseudomonas aeruginosa PAK

36

100

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362

Multiple sequence alignment