Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GM160_RS10520 Genome accession   NZ_CP046415
Coordinates   2326100..2326543 (-) Length   147 a.a.
NCBI ID   WP_136867377.1    Uniprot ID   A0A4U0YFC9
Organism   Guyparkeria halophila strain sp2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2310316..2325824 2326100..2326543 flank 276


Gene organization within MGE regions


Location: 2310316..2326543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM160_RS10445 (GM160_10445) - 2310316..2310585 (+) 270 WP_136867351.1 hypothetical protein -
  GM160_RS10450 (GM160_10450) - 2310623..2311069 (+) 447 WP_136867350.1 hypothetical protein -
  GM160_RS10455 (GM160_10455) - 2311206..2311910 (+) 705 WP_156574991.1 outer membrane protein -
  GM160_RS10460 (GM160_10460) - 2312008..2312712 (+) 705 WP_156574993.1 hypothetical protein -
  GM160_RS10465 (GM160_10465) - 2312774..2313355 (+) 582 WP_231505767.1 hypothetical protein -
  GM160_RS10470 (GM160_10470) - 2313352..2314587 (+) 1236 WP_197039129.1 AmpG family muropeptide MFS transporter -
  GM160_RS10475 (GM160_10475) - 2314676..2315158 (+) 483 WP_156574995.1 hypothetical protein -
  GM160_RS10480 (GM160_10480) - 2315288..2316085 (+) 798 WP_156574997.1 ProQ/FINO family protein -
  GM160_RS10485 (GM160_10485) mtgA 2316613..2317338 (+) 726 WP_156574999.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GM160_RS10490 (GM160_10490) - 2317391..2318716 (-) 1326 WP_156575001.1 flippase -
  GM160_RS10495 (GM160_10495) - 2318716..2319573 (-) 858 WP_156575003.1 polysialyltransferase family glycosyltransferase -
  GM160_RS10500 (GM160_10500) - 2319844..2320989 (-) 1146 WP_156575005.1 UDP-N-acetylglucosamine 2-epimerase -
  GM160_RS10505 (GM160_10505) - 2321057..2321935 (-) 879 WP_156575007.1 glycosyltransferase family 2 protein -
  GM160_RS10510 (GM160_10510) - 2322109..2324118 (-) 2010 WP_156575009.1 tetratricopeptide repeat protein -
  GM160_RS10515 (GM160_10515) - 2324118..2326034 (-) 1917 WP_197039130.1 tetratricopeptide repeat protein -
  GM160_RS10520 (GM160_10520) pilA 2326100..2326543 (-) 444 WP_136867377.1 pilin Machinery gene

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15189.20 Da        Isoelectric Point: 4.9429

>NTDB_id=404182 GM160_RS10520 WP_136867377.1 2326100..2326543(-) (pilA) [Guyparkeria halophila strain sp2]
MNKQVQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAQVSEGMSLSSGAKTAVAEFYNQRGSFPTSNASAGLSTAASI
SGEYVSQVNVAQGGTAGLIGATFNTGNENTNITGDILLISAIDQGGSISWTCKSTTLADKYIPASCR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=404182 GM160_RS10520 WP_136867377.1 2326100..2326543(-) (pilA) [Guyparkeria halophila strain sp2]
ATGAACAAGCAGGTTCAGAAGGGTTTCACCCTGATTGAACTGATGATCGTGGTCGCGATCATCGGCATCCTCGCCGCAAT
TGCCATTCCGGCTTACCAGGATTACACGATCCGTGCTCAAGTTTCGGAAGGGATGAGCTTGAGTTCGGGAGCAAAGACGG
CTGTCGCTGAATTCTATAATCAGCGCGGTTCGTTCCCCACGAGTAATGCCTCTGCGGGGCTTTCTACCGCAGCGAGCATC
TCCGGGGAATATGTAAGCCAAGTTAACGTTGCTCAAGGTGGTACAGCCGGGCTCATCGGTGCAACCTTTAACACCGGTAA
TGAAAACACCAACATCACCGGAGATATCCTTCTGATCTCCGCGATTGATCAAGGTGGTTCGATTTCGTGGACCTGCAAGT
CCACCACTCTGGCAGATAAGTACATTCCGGCCAGCTGCCGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(38-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U0YFC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

42.69

100

0.497
  pilE Neisseria gonorrhoeae strain FA1090

45.283

100

0.49
  pilE Neisseria gonorrhoeae MS11

44.375

100

0.483
  pilA/pilA1 Eikenella corrodens VA1

43.59

100

0.463
  pilA2 Legionella pneumophila strain ERS1305867

47.183

96.599

0.456
  pilA2 Legionella pneumophila str. Paris

47.183

96.599

0.456
  comP Acinetobacter baylyi ADP1

42.568

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.509

100

0.422
  pilA Vibrio cholerae strain A1552

40.667

100

0.415
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.667

100

0.415
  pilA Vibrio cholerae C6706

40.667

100

0.415
  pilA Acinetobacter baumannii strain A118

40.845

96.599

0.395
  pilA Pseudomonas aeruginosa PAK

36.842

100

0.381

Multiple sequence alignment