Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   GLO74_RS01010 Genome accession   NZ_CP046354
Coordinates   202276..203217 (+) Length   313 a.a.
NCBI ID   WP_000249555.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 310     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 197276..208217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLO74_RS00990 (GLO74_01010) - 197343..199160 (+) 1818 WP_001220846.1 acyltransferase family protein -
  GLO74_RS00995 (GLO74_01015) nagA 199313..200464 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  GLO74_RS01000 (GLO74_01020) - 200627..201685 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  GLO74_RS01005 (GLO74_01025) - 201836..202201 (+) 366 WP_000286415.1 DUF1033 family protein -
  GLO74_RS01010 (GLO74_01030) comGA/cglA/cilD 202276..203217 (+) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  GLO74_RS01015 (GLO74_01035) comGB/cglB 203165..204181 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  GLO74_RS01020 (GLO74_01040) comGC/cglC 204183..204509 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  GLO74_RS01025 (GLO74_01045) comGD/cglD 204502..204906 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  GLO74_RS01030 (GLO74_01050) comGE/cglE 204869..205171 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  GLO74_RS01035 (GLO74_01055) comGF/cglF 205134..205595 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  GLO74_RS01040 (GLO74_01060) comGG/cglG 205573..205965 (+) 393 WP_001818028.1 competence type IV pilus minor pilin ComGG Machinery gene
  GLO74_RS01045 (GLO74_01065) - 206121..206708 (+) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  GLO74_RS01050 (GLO74_01075) comYH 206769..207722 (+) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.49 Da        Isoelectric Point: 6.0083

>NTDB_id=402928 GLO74_RS01010 WP_000249555.1 202276..203217(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 310]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=402928 GLO74_RS01010 WP_000249555.1 202276..203217(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 310]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment