Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   GLO74_RS01015 Genome accession   NZ_CP046354
Coordinates   203165..204181 (+) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 310     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 198165..209181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLO74_RS00995 (GLO74_01015) nagA 199313..200464 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  GLO74_RS01000 (GLO74_01020) - 200627..201685 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  GLO74_RS01005 (GLO74_01025) - 201836..202201 (+) 366 WP_000286415.1 DUF1033 family protein -
  GLO74_RS01010 (GLO74_01030) comGA/cglA/cilD 202276..203217 (+) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  GLO74_RS01015 (GLO74_01035) comGB/cglB 203165..204181 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  GLO74_RS01020 (GLO74_01040) comGC/cglC 204183..204509 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  GLO74_RS01025 (GLO74_01045) comGD/cglD 204502..204906 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  GLO74_RS01030 (GLO74_01050) comGE/cglE 204869..205171 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  GLO74_RS01035 (GLO74_01055) comGF/cglF 205134..205595 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  GLO74_RS01040 (GLO74_01060) comGG/cglG 205573..205965 (+) 393 WP_001818028.1 competence type IV pilus minor pilin ComGG Machinery gene
  GLO74_RS01045 (GLO74_01065) - 206121..206708 (+) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  GLO74_RS01050 (GLO74_01075) comYH 206769..207722 (+) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  GLO74_RS01055 (GLO74_01080) - 207773..208963 (+) 1191 WP_000167765.1 acetate kinase -
  GLO74_RS11245 - 208964..209095 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=402929 GLO74_RS01015 WP_074017570.1 203165..204181(+) (comGB/cglB) [Streptococcus pneumoniae strain 310]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=402929 GLO74_RS01015 WP_074017570.1 203165..204181(+) (comGB/cglB) [Streptococcus pneumoniae strain 310]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment