Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   GLO74_RS01030 Genome accession   NZ_CP046354
Coordinates   204869..205171 (+) Length   100 a.a.
NCBI ID   WP_000413382.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 310     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 199869..210171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLO74_RS01000 (GLO74_01020) - 200627..201685 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  GLO74_RS01005 (GLO74_01025) - 201836..202201 (+) 366 WP_000286415.1 DUF1033 family protein -
  GLO74_RS01010 (GLO74_01030) comGA/cglA/cilD 202276..203217 (+) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  GLO74_RS01015 (GLO74_01035) comGB/cglB 203165..204181 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  GLO74_RS01020 (GLO74_01040) comGC/cglC 204183..204509 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  GLO74_RS01025 (GLO74_01045) comGD/cglD 204502..204906 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  GLO74_RS01030 (GLO74_01050) comGE/cglE 204869..205171 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  GLO74_RS01035 (GLO74_01055) comGF/cglF 205134..205595 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  GLO74_RS01040 (GLO74_01060) comGG/cglG 205573..205965 (+) 393 WP_001818028.1 competence type IV pilus minor pilin ComGG Machinery gene
  GLO74_RS01045 (GLO74_01065) - 206121..206708 (+) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  GLO74_RS01050 (GLO74_01075) comYH 206769..207722 (+) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  GLO74_RS01055 (GLO74_01080) - 207773..208963 (+) 1191 WP_000167765.1 acetate kinase -
  GLO74_RS11245 - 208964..209095 (+) 132 WP_000768904.1 hypothetical protein -
  GLO74_RS01060 (GLO74_01085) rnpA 209112..209483 (+) 372 WP_000739247.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11108.08 Da        Isoelectric Point: 9.7273

>NTDB_id=402932 GLO74_RS01030 WP_000413382.1 204869..205171(+) (comGE/cglE) [Streptococcus pneumoniae strain 310]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=402932 GLO74_RS01030 WP_000413382.1 204869..205171(+) (comGE/cglE) [Streptococcus pneumoniae strain 310]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCAGTAGTCGCTCTAGCTATCTT
TGCCAGCATTGCGACCCTCCTTTTGGGACAAATTCAAAAAAATAGGCAAGAGGAAGCAAAAATCTTGCAAAAGGAAGAAG
TCTTGAGGGTAGCTAAGATGGCCCTGCAGACGGGGCAAAATCAGGTAAGCATCAACGGAGTTGAGATTCAGGTATTTTCT
AGTGAAAAAGGATTGGAGGTCTACCATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus pneumoniae Rx1

100

100

1
  comGE/cglE Streptococcus pneumoniae D39

100

100

1
  comGE/cglE Streptococcus pneumoniae R6

100

100

1
  comGE/cglE Streptococcus pneumoniae TIGR4

100

100

1
  comGE/cglE Streptococcus mitis NCTC 12261

98

100

0.98
  comGE/cglE Streptococcus mitis SK321

98

100

0.98
  comYE Streptococcus mutans UA140

44.444

90

0.4
  comYE Streptococcus mutans UA159

44.444

90

0.4

Multiple sequence alignment