Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   GJV52_RS07695 Genome accession   NZ_CP046027
Coordinates   1507765..1508208 (+) Length   147 a.a.
NCBI ID   WP_100564486.1    Uniprot ID   -
Organism   Neisseria brasiliensis strain N.177.16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1502266..1507434 1507765..1508208 flank 331


Gene organization within MGE regions


Location: 1502266..1508208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJV52_RS07665 (GJV52_07665) tnpA 1502266..1502682 (-) 417 WP_095503171.1 IS200/IS605 family transposase -
  GJV52_RS07670 (GJV52_07670) - 1502727..1503869 (+) 1143 WP_095503172.1 RNA-guided endonuclease TnpB family protein -
  GJV52_RS07680 (GJV52_07680) - 1504482..1505273 (+) 792 WP_100564634.1 fimb protein -
  GJV52_RS07685 (GJV52_07685) - 1505831..1506973 (-) 1143 WP_095503172.1 RNA-guided endonuclease TnpB family protein -
  GJV52_RS07690 (GJV52_07690) tnpA 1507018..1507434 (+) 417 WP_095503171.1 IS200/IS605 family transposase -
  GJV52_RS07695 (GJV52_07695) pilE 1507765..1508208 (+) 444 WP_100564486.1 pilin Machinery gene

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15515.10 Da        Isoelectric Point: 9.5874

>NTDB_id=400320 GJV52_RS07695 WP_100564486.1 1507765..1508208(+) (pilE) [Neisseria brasiliensis strain N.177.16]
MKAIQKGFTLIELMIVIAIIGILAVIALPAYQDYTGRAQVSEAIGLMEGQKSAVVEYYADKGKWPTKNEDAGIADATAIT
GKYTAKVTVEGKDGVITATMKSAGVNKDVKGKTVILVPKQEAGSFSWTCKSGAKDGLPAKFLPSSCR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=400320 GJV52_RS07695 WP_100564486.1 1507765..1508208(+) (pilE) [Neisseria brasiliensis strain N.177.16]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGTTGATGATCGTAATCGCAATTATCGGTATTTTGGCCGTAATCGC
TCTGCCAGCATACCAAGACTATACTGGTCGTGCCCAAGTTTCTGAAGCTATTGGCTTGATGGAAGGTCAAAAATCTGCAG
TAGTAGAATATTATGCTGATAAAGGTAAATGGCCTACTAAAAATGAAGATGCTGGTATTGCAGATGCTACTGCTATTACT
GGTAAATATACTGCAAAAGTAACAGTAGAAGGCAAAGATGGTGTTATTACTGCAACTATGAAATCTGCTGGTGTAAATAA
AGATGTTAAAGGGAAGACAGTTATCTTAGTGCCTAAGCAAGAAGCTGGTTCATTTTCTTGGACTTGTAAATCAGGTGCTA
AAGATGGTTTACCAGCTAAATTCTTGCCATCATCTTGTCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

57.764

100

0.633
  pilE Neisseria gonorrhoeae strain FA1090

55

100

0.599
  pilA/pilA1 Eikenella corrodens VA1

45.57

100

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.234

100

0.476
  pilA Ralstonia pseudosolanacearum GMI1000

38.095

100

0.435
  pilA2 Legionella pneumophila strain ERS1305867

44.444

97.959

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.722

100

0.429
  pilA2 Legionella pneumophila str. Paris

43.75

97.959

0.429
  pilA Pseudomonas aeruginosa PAK

36.943

100

0.395
  pilA Acinetobacter baumannii strain A118

39.583

97.959

0.388
  comP Acinetobacter baylyi ADP1

37.748

100

0.388
  pilA Acinetobacter nosocomialis M2

39.437

96.599

0.381
  pilA Haemophilus influenzae 86-028NP

36.054

100

0.361

Multiple sequence alignment