Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GII14_RS16050 Genome accession   NZ_CP045857
Coordinates   3533903..3535015 (-) Length   370 a.a.
NCBI ID   WP_165565433.1    Uniprot ID   -
Organism   Shewanella chilikensis strain DC57     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3528903..3540015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII14_RS16030 (GII14_16040) - 3530030..3530764 (-) 735 WP_165565430.1 tryptophan 2,3-dioxygenase family protein -
  GII14_RS16035 (GII14_16045) hemH 3530936..3531949 (-) 1014 WP_165565431.1 ferrochelatase -
  GII14_RS16040 (GII14_16050) - 3531984..3532475 (-) 492 WP_224866272.1 glutathione peroxidase -
  GII14_RS16045 (GII14_16055) - 3532638..3533747 (-) 1110 WP_165565432.1 hypothetical protein -
  GII14_RS16050 (GII14_16060) pilU 3533903..3535015 (-) 1113 WP_165565433.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GII14_RS16055 (GII14_16065) pilT 3535025..3536062 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  GII14_RS16060 (GII14_16070) - 3536099..3536794 (+) 696 WP_165565434.1 YggS family pyridoxal phosphate-dependent enzyme -
  GII14_RS16065 (GII14_16075) proC 3536927..3537745 (+) 819 WP_101055491.1 pyrroline-5-carboxylate reductase -
  GII14_RS16070 (GII14_16080) - 3537769..3538320 (+) 552 WP_101055490.1 YggT family protein -
  GII14_RS16075 (GII14_16085) yggU 3538320..3538607 (+) 288 WP_037436422.1 DUF167 family protein YggU -
  GII14_RS16080 (GII14_16090) - 3538670..3539104 (+) 435 WP_101055484.1 DUF4426 domain-containing protein -
  GII14_RS16085 (GII14_16095) rdgB 3539324..3539926 (+) 603 WP_101055482.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3711

>NTDB_id=398695 GII14_RS16050 WP_165565433.1 3533903..3535015(-) (pilU) [Shewanella chilikensis strain DC57]
MDVRPFLKAMVERKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEVLINTPRVASLIQKNELHVLKETMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=398695 GII14_RS16050 WP_165565433.1 3533903..3535015(-) (pilU) [Shewanella chilikensis strain DC57]
ATGGACGTCAGACCTTTTCTCAAGGCTATGGTGGAGCGCAAGGCCTCGGATCTCTTTATTACCGCGGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTCGAATCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGTGAATGTAACTTTGCCGTGGGCGCCAAGGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCAGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCGACAGTGGA
GCAGTTGTACCTGCCGCCCATTCTCAAAGACTTGGTGATGAGCAAGCGCGGATTGGTGATCATGGTCGGGGGAACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTATCGCAATGCCAATGCCAGGGGACATATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACATGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAATCTTTTGATGC
AGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGATGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CTCTGTCGTTCGCTGAAACCGGCCATTTGTGTATGGCAACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCTGAGAGTAAGCACCAACAGCTGTTGTTTGACCTGTCGCTTAACCTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGCGGACGGCACTGGTCGGCGCGCAGCCATCGAAGTGCTTATCAACACTCCCAGAGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGAGACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGATCAGGCA
CTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCTGACTCGCCTAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAGGGAGTCACCCTGGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

59.943

95.135

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Acinetobacter nosocomialis M2

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment