Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIU96_RS02165 Genome accession   NZ_CP045367
Coordinates   479450..480571 (+) Length   373 a.a.
NCBI ID   WP_061332594.1    Uniprot ID   -
Organism   Marinobacter sp. THAF39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 419485..486319 479450..480571 within 0


Gene organization within MGE regions


Location: 419485..486319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU96_RS01890 (FIU96_01920) - 419587..420426 (-) 840 WP_152437179.1 uroporphyrinogen-III synthase -
  FIU96_RS01895 (FIU96_01925) hemC 420407..421348 (-) 942 WP_152437180.1 hydroxymethylbilane synthase -
  FIU96_RS01900 (FIU96_01930) - 421434..422189 (-) 756 WP_061332519.1 LytR/AlgR family response regulator transcription factor -
  FIU96_RS01905 (FIU96_01935) - 422220..423308 (-) 1089 WP_416376904.1 sensor histidine kinase -
  FIU96_RS01910 (FIU96_01940) argH 423368..424783 (+) 1416 WP_152437182.1 argininosuccinate lyase -
  FIU96_RS20070 - 424926..426020 (+) 1095 WP_228715476.1 hypothetical protein -
  FIU96_RS20075 - 426106..427008 (+) 903 WP_267313115.1 phospholipase effector Tle1 domain-containing protein -
  FIU96_RS01920 (FIU96_01955) - 427005..427610 (+) 606 WP_323847459.1 DUF2931 family protein -
  FIU96_RS01925 (FIU96_01960) - 427645..428250 (+) 606 WP_323847460.1 DUF2931 family protein -
  FIU96_RS01930 (FIU96_01965) - 428288..428890 (+) 603 WP_152437183.1 DUF2931 family protein -
  FIU96_RS01935 (FIU96_01970) - 428909..431350 (-) 2442 WP_152437184.1 EAL domain-containing protein -
  FIU96_RS01940 (FIU96_01975) - 431490..434339 (+) 2850 WP_152437185.1 class I adenylate cyclase -
  FIU96_RS01945 (FIU96_01980) lptM 434412..434582 (+) 171 WP_228715477.1 LPS translocon maturation chaperone LptM -
  FIU96_RS01950 (FIU96_01985) lysA 434627..435877 (+) 1251 WP_061332524.1 diaminopimelate decarboxylase -
  FIU96_RS01955 (FIU96_01990) dapF 435892..436806 (+) 915 WP_152437187.1 diaminopimelate epimerase -
  FIU96_RS01960 (FIU96_01995) - 436824..437576 (+) 753 WP_061332525.1 DUF484 family protein -
  FIU96_RS01965 (FIU96_02000) xerC 437612..438544 (+) 933 WP_152439571.1 tyrosine recombinase XerC -
  FIU96_RS01970 (FIU96_02005) - 438556..439950 (+) 1395 WP_152437188.1 GGDEF domain-containing protein -
  FIU96_RS01975 (FIU96_02010) - 439960..440193 (-) 234 WP_061332527.1 DUF2789 domain-containing protein -
  FIU96_RS01980 (FIU96_02015) - 440291..443047 (+) 2757 WP_152480524.1 ATP-binding protein -
  FIU96_RS01985 (FIU96_02020) - 443061..443426 (-) 366 WP_072678634.1 hypothetical protein -
  FIU96_RS01990 (FIU96_02025) - 443658..444266 (+) 609 WP_152437190.1 hypothetical protein -
  FIU96_RS01995 (FIU96_02030) - 444406..446484 (+) 2079 WP_267313154.1 patatin-like phospholipase family protein -
  FIU96_RS02000 (FIU96_02035) - 446505..447167 (-) 663 WP_152437192.1 GntR family transcriptional regulator -
  FIU96_RS02005 (FIU96_02040) cls 447245..448681 (-) 1437 WP_152437193.1 cardiolipin synthase -
  FIU96_RS02010 (FIU96_02045) - 448678..449718 (-) 1041 WP_058091334.1 AraC family transcriptional regulator -
  FIU96_RS02015 (FIU96_02055) - 450106..450897 (+) 792 WP_152437194.1 alpha/beta fold hydrolase -
  FIU96_RS02020 (FIU96_02060) - 451000..452085 (+) 1086 WP_152439572.1 SMP-30/gluconolactonase/LRE family protein -
  FIU96_RS02025 (FIU96_02065) - 452082..452546 (+) 465 WP_058091336.1 acyl-CoA thioesterase -
  FIU96_RS02030 (FIU96_02070) - 452624..453604 (+) 981 WP_152437195.1 NADP-dependent oxidoreductase -
  FIU96_RS02035 (FIU96_02075) tcdA 453601..454398 (-) 798 WP_061332537.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
  FIU96_RS02040 (FIU96_02080) - 454657..456432 (-) 1776 WP_072678623.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -
  FIU96_RS02045 (FIU96_02085) - 456609..457343 (+) 735 WP_152437196.1 SDR family oxidoreductase -
  FIU96_RS02050 (FIU96_02090) - 457362..458306 (-) 945 WP_072678622.1 sodium-dependent bicarbonate transport family permease -
  FIU96_RS02055 (FIU96_02095) - 458481..459119 (-) 639 WP_152437197.1 ChrR family anti-sigma-E factor -
  FIU96_RS02060 (FIU96_02100) - 459116..459715 (-) 600 WP_152437198.1 sigma-70 family RNA polymerase sigma factor -
  FIU96_RS02065 (FIU96_02105) - 459944..460990 (+) 1047 WP_152437199.1 AraC family transcriptional regulator -
  FIU96_RS02070 (FIU96_02110) speA 460994..462904 (-) 1911 WP_152437200.1 biosynthetic arginine decarboxylase -
  FIU96_RS02075 (FIU96_02115) speE 463134..464015 (+) 882 WP_061332546.1 polyamine aminopropyltransferase -
  FIU96_RS02080 (FIU96_02120) - 464108..464302 (+) 195 WP_061332547.1 DUF6316 family protein -
  FIU96_RS02085 (FIU96_02125) - 464379..465626 (-) 1248 WP_152437201.1 ammonium transporter -
  FIU96_RS02090 (FIU96_02130) glnK 465698..466036 (-) 339 WP_058091350.1 P-II family nitrogen regulator -
  FIU96_RS02095 (FIU96_02135) - 466421..466672 (+) 252 WP_061332549.1 accessory factor UbiK family protein -
  FIU96_RS02100 (FIU96_02140) - 466746..468272 (+) 1527 WP_152437202.1 YifB family Mg chelatase-like AAA ATPase -
  FIU96_RS02105 (FIU96_02145) trmB 468345..469070 (+) 726 WP_061332551.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  FIU96_RS02110 (FIU96_02150) - 469083..469631 (-) 549 WP_061332552.1 hypothetical protein -
  FIU96_RS02115 (FIU96_02155) hemW 469631..470776 (-) 1146 WP_152437203.1 radical SAM family heme chaperone HemW -
  FIU96_RS02120 (FIU96_02160) rdgB 470781..471380 (-) 600 WP_061332554.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FIU96_RS02125 (FIU96_02165) - 471385..471831 (-) 447 WP_152439573.1 DUF4426 domain-containing protein -
  FIU96_RS02130 (FIU96_02170) metW 471831..472433 (-) 603 WP_061332555.1 methionine biosynthesis protein MetW -
  FIU96_RS02135 (FIU96_02175) metX 472430..473578 (-) 1149 WP_061332556.1 homoserine O-succinyltransferase MetX -
  FIU96_RS02140 (FIU96_02180) - 473751..475715 (-) 1965 WP_152437204.1 dynamin family protein -
  FIU96_RS02145 (FIU96_02185) - 475922..476506 (-) 585 WP_061332558.1 YggT family protein -
  FIU96_RS02150 (FIU96_02190) proC 476529..477362 (-) 834 WP_061332559.1 pyrroline-5-carboxylate reductase -
  FIU96_RS02155 (FIU96_02195) - 477426..478139 (-) 714 WP_152437205.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU96_RS02160 (FIU96_02200) pilT 478192..479226 (+) 1035 WP_058091364.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU96_RS02165 (FIU96_02205) pilU 479450..480571 (+) 1122 WP_061332594.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU96_RS02170 (FIU96_02210) - 480730..482103 (+) 1374 WP_152437206.1 OmpP1/FadL family transporter -
  FIU96_RS02175 (FIU96_02215) - 482143..482496 (-) 354 WP_152437207.1 hypothetical protein -
  FIU96_RS02180 (FIU96_02220) polA 482694..485426 (-) 2733 WP_152437208.1 DNA polymerase I -
  FIU96_RS02185 (FIU96_02225) - 485512..485847 (+) 336 WP_061332562.1 DUF2782 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41494.73 Da        Isoelectric Point: 6.6618

>NTDB_id=393668 FIU96_RS02165 WP_061332594.1 479450..480571(+) (pilU) [Marinobacter sp. THAF39]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLALPEIVKDLAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLRAIVAQQLVPTPDGKGRKAVIEVLINTPLVADLIRKAEVHKLKELMAKSNESGMQTFDQA
LYRLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLTIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=393668 FIU96_RS02165 WP_061332594.1 479450..480571(+) (pilU) [Marinobacter sp. THAF39]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAAAAAGGCGGATCGGACCTGTTCATTACAGCGGGTGTACCGCCAAG
CATGAAGGTGAACGGGAAAGTGCTCCCTGTGACCAAAAACGCGCTGACGCCGGAGCAGACGAGGGAGTTTGTCTACGGCG
CCATGAACGACAAACAGCGCGCCGAATTCGAAGAAACCCACGAATGTAACTTCGCCATCAGCGCCCGCGGCATTGGCCGC
TTCCGGGTGTCCGCGTTTTTCCAACGCAACCTGTGCGGTATGGTATTGCGACGGATTGAAGTGAAAATTCCCCAGATTGA
CGACCTGGCGCTGCCCGAGATTGTCAAAGACCTGGCCATGACCAAACGTGGCCTGATCATGTTTGTGGGCGCCACCGGTA
CCGGTAAGTCCACCTCCCTGGCCGCCATGCTCGGCCATCGGAACCGTAACAGCCGCGGCCACATCATCTCCATCGAAGAT
CCTATCGAATTCGTGCACCAGCACCAGGGCTGCATCGTCACCCAGCGGGAGGTGGGCATCGATACTGAAAGCTTCGAAGT
GGCCCTGAAAAATACTCTGCGACAGGCGCCGGACGTTATCCTGATTGGTGAGGTGCGTACCCGCCAGACCATGGAATATT
CGGTACAGTTCGCGGAAACCGGCCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGGATT
ATCCAGTTCTTCCCGCCAGAACAGCACAACCAGATCTGGATGGACCTCTCCCTGAACCTCCGCGCCATAGTGGCTCAGCA
GCTGGTGCCGACGCCGGATGGCAAAGGCCGTAAGGCCGTAATCGAAGTATTGATCAATACGCCGCTGGTGGCGGACCTGA
TTCGTAAAGCCGAAGTGCACAAACTCAAAGAGTTGATGGCCAAGTCCAACGAGTCCGGCATGCAGACGTTTGACCAGGCG
TTGTACCGGCTGTACGCCGAAGGCTCCATCACCTATGAAGACGCTTTGGCCCACGCGGATTCCGCCAACGATCTGCGCCT
GATGATCAAGCTCGGTGCCGATGCCCAGGGCGCCGACAAGCTGTCATCGTCGGTCGACAAGCTCACCATTCAGGACGATT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.976

99.464

0.786
  pilU Acinetobacter baylyi ADP1

64.607

95.442

0.617
  pilU Vibrio cholerae strain A1552

56.757

99.196

0.563
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.773

90.885

0.389
  pilT Acinetobacter baumannii D1279779

41.194

89.812

0.37
  pilT Acinetobacter baumannii strain A118

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

40.896

89.812

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.909

88.472

0.362
  pilT Vibrio cholerae strain A1552

40.909

88.472

0.362

Multiple sequence alignment