Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NLA_RS07610 Genome accession   NC_014752
Coordinates   1590976..1592106 (-) Length   376 a.a.
NCBI ID   WP_042508370.1    Uniprot ID   -
Organism   Neisseria lactamica 020-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1585976..1597106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA_RS07575 (NLA_14540) - 1586641..1587063 (-) 423 WP_013449237.1 protein Gly1ORF1 -
  NLA_RS07580 (NLA_14550) - 1587199..1587516 (-) 318 WP_013449238.1 DUF2322 family protein -
  NLA_RS07585 (NLA_14560) upp 1587539..1588165 (-) 627 WP_002242442.1 uracil phosphoribosyltransferase -
  NLA_RS07590 (NLA_14570) grxD 1588271..1588582 (-) 312 WP_013449239.1 Grx4 family monothiol glutaredoxin -
  NLA_RS07595 (NLA_14590) - 1588826..1589605 (-) 780 WP_013449240.1 TatD family hydrolase -
  NLA_RS07600 (NLA_14600) - 1589610..1589960 (-) 351 WP_003708068.1 PilZ domain-containing protein -
  NLA_RS07605 (NLA_14610) - 1589964..1590941 (-) 978 WP_013449241.1 DNA polymerase III subunit delta' -
  NLA_RS07610 (NLA_14620) pilU 1590976..1592106 (-) 1131 WP_042508370.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NLA_RS07615 (NLA_14630) - 1592272..1592973 (+) 702 WP_013449243.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NLA_RS07620 (NLA_14640) lepA 1593118..1594911 (+) 1794 WP_013449244.1 translation elongation factor 4 -
  NLA_RS07625 (NLA_14650) lepB 1595056..1596075 (+) 1020 WP_013449245.1 signal peptidase I -
  NLA_RS13185 - 1596294..1596443 (-) 150 WP_003708054.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42143.30 Da        Isoelectric Point: 6.5758

>NTDB_id=39074 NLA_RS07610 WP_042508370.1 1590976..1592106(-) (pilU) [Neisseria lactamica 020-06]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDTPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQDALKNADSAHDLRLAVQLRSRRAQSSGPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=39074 NLA_RS07610 WP_042508370.1 1590976..1592106(-) (pilU) [Neisseria lactamica 020-06]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCCTGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCGATGAAGCTGGACGGCAAAATTACCCGCATCACGGATACGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTCAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTGATTTTTGTCGGCGGTACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAATTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGGCAGGCACCCGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTAATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCGGCGCATGATTTGCGTTTGGCTGTTCAGTTGAGAAGCCGCCGCGCGCAAAGTTCCGGACCGGATTTGG
AATTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.943

93.617

0.561
  pilU Acinetobacter baylyi ADP1

58.017

91.223

0.529
  pilU Vibrio cholerae strain A1552

54.039

95.479

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.059

93.883

0.404
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment