Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   XOCgx_RS05765 Genome accession   NZ_CP043403
Coordinates   1197838..1198263 (+) Length   141 a.a.
NCBI ID   WP_047340102.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzicola strain GX01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1182077..1200628 1197838..1198263 within 0


Gene organization within MGE regions


Location: 1182077..1200628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XOCgx_RS05695 (XOCgx_1241) - 1182077..1182268 (-) 192 WP_149621391.1 hypothetical protein -
  XOCgx_RS23280 (XOCgx_1242) - 1182661..1183146 (-) 486 WP_154219727.1 DUF6714 family protein -
  XOCgx_RS05705 (XOCgx_1243) - 1183146..1183778 (-) 633 WP_224229828.1 hypothetical protein -
  XOCgx_RS05710 (XOCgx_1244) - 1183777..1184793 (+) 1017 WP_076342622.1 IS30 family transposase -
  XOCgx_RS24310 (XOCgx_1245) - 1184903..1185433 (-) 531 Protein_1027 transposase -
  XOCgx_RS05730 (XOCgx_1248) - 1186663..1187031 (-) 369 WP_011408064.1 DUF6869 domain-containing protein -
  XOCgx_RS05735 (XOCgx_1249) - 1187037..1188620 (-) 1584 WP_224229885.1 RHS repeat-associated core domain-containing protein -
  XOCgx_RS05740 (XOCgx_1251) - 1189025..1193524 (-) 4500 WP_224229830.1 RHS repeat-associated core domain-containing protein -
  XOCgx_RS05750 (XOCgx_1253) coaE 1194733..1195341 (-) 609 WP_014504326.1 dephospho-CoA kinase -
  XOCgx_RS05755 (XOCgx_1254) - 1195355..1196218 (-) 864 WP_047340103.1 A24 family peptidase -
  XOCgx_RS05760 (XOCgx_1255) pilC 1196225..1197484 (-) 1260 WP_014504324.1 type II secretion system F family protein Machinery gene
  XOCgx_RS05765 (XOCgx_1256) pilA2 1197838..1198263 (+) 426 WP_047340102.1 pilin Machinery gene
  XOCgx_RS05770 (XOCgx_1257) - 1198399..1199988 (+) 1590 WP_014504322.1 phosphoethanolamine transferase -
  XOCgx_RS05775 (XOCgx_1258) - 1199999..1200628 (+) 630 WP_024711608.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14401.63 Da        Isoelectric Point: 9.0090

>NTDB_id=383465 XOCgx_RS05765 WP_047340102.1 1197838..1198263(+) (pilA2) [Xanthomonas oryzae pv. oryzicola strain GX01]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVAETSSSLGGVANVTLANSGYAFPGQTK
YVSAVTIASGTGKVTVTSTVPNATGDIVLTPTDVGGGQLKWTCSSAIATKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=383465 XOCgx_RS05765 WP_047340102.1 1197838..1198263(+) (pilA2) [Xanthomonas oryzae pv. oryzicola strain GX01]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCACCTGCCAAGCTTGCGGTAG
CGGAGACTTCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACGCATTCCCGGGTCAGACGAAG
TATGTCAGCGCCGTCACTATCGCCAGTGGCACGGGTAAAGTCACTGTGACCTCCACGGTGCCCAACGCTACCGGTGACAT
CGTGCTGACCCCGACTGACGTCGGTGGTGGCCAGTTGAAGTGGACCTGCTCTTCCGCCATCGCCACCAAGTACCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50

96.454

0.482
  pilA Ralstonia pseudosolanacearum GMI1000

40.854

100

0.475
  pilA2 Legionella pneumophila strain ERS1305867

49.265

96.454

0.475
  comP Acinetobacter baylyi ADP1

42.857

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

39.241

100

0.44
  pilE Neisseria gonorrhoeae MS11

37.037

100

0.426
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.58

97.872

0.397
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.741

95.745

0.39
  pilA Acinetobacter baumannii strain A118

36.879

100

0.369
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362

Multiple sequence alignment