Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M301_RS13590 Genome accession   NC_014207
Coordinates   2952770..2953936 (+) Length   388 a.a.
NCBI ID   WP_013149360.1    Uniprot ID   D7DP24
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2947770..2958936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS14630 (M301_2694) - 2948634..2948798 (+) 165 WP_013149354.1 hypothetical protein -
  M301_RS13560 (M301_2696) - 2949357..2949863 (+) 507 WP_013149356.1 DUF1993 domain-containing protein -
  M301_RS13565 (M301_2697) - 2949932..2951281 (-) 1350 WP_013149357.1 NRAMP family divalent metal transporter -
  M301_RS13570 (M301_2698) - 2951568..2951804 (+) 237 WP_041359534.1 hypothetical protein -
  M301_RS14635 (M301_2699) - 2952287..2952568 (-) 282 WP_013149359.1 hypothetical protein -
  M301_RS13590 (M301_2700) pilU 2952770..2953936 (+) 1167 WP_013149360.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M301_RS13595 (M301_2701) - 2953966..2954403 (-) 438 WP_013149361.1 DUF2721 domain-containing protein -
  M301_RS13605 (M301_2702) - 2954872..2955744 (+) 873 WP_041360255.1 DMT family transporter -
  M301_RS13610 (M301_2703) - 2955850..2956029 (+) 180 WP_013149363.1 hypothetical protein -
  M301_RS14735 - 2956389..2956574 (+) 186 WP_238524646.1 hypothetical protein -
  M301_RS14740 - 2956638..2957006 (+) 369 Protein_2688 MOSC domain-containing protein -
  M301_RS13625 (M301_2705) - 2957503..2958603 (+) 1101 WP_013149364.1 porin -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43294.96 Da        Isoelectric Point: 6.6637

>NTDB_id=37398 M301_RS13590 WP_013149360.1 2952770..2953936(+) (pilU) [Methylotenera versatilis 301]
MTNDQALTYMHNLLRAMLDKKASDLFISADFPPAMKIDGKMTPVTQQKLTGELTKAFADAIMNDKQKAEFAVEKECNFAI
WPKEIGRFRVNVFLQQEKIGMVLRTITTKIPNFEDLGLPKVLKEVMMTKRGLVILVGGTGSGKSTTLAALIDYRNEHSFG
HIITVEDPVEYVHQSKNCLVTHREVGRDTNGWFNALKNTLRQAPDVILIGEIRDRETMEFALAFAETGHLCMATLHANSA
NQALDRIINFFPEERHAQLHMDLSLNLRAFVSQRLVSKVGGGRSAAVEILLNSPLISDLILRGETGMVKEIMAKSTEQGM
QTFDQALFQLCEDGVISEEDALRNADSLTELRLRFKLKGKNAGAKSTSMFALHEEPKEEKAPEVQAAM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=37398 M301_RS13590 WP_013149360.1 2952770..2953936(+) (pilU) [Methylotenera versatilis 301]
ATGACAAACGACCAAGCGCTGACCTATATGCACAATCTTTTACGTGCCATGTTAGACAAAAAAGCATCAGACTTATTCAT
CTCTGCAGATTTTCCACCAGCAATGAAAATAGATGGAAAAATGACGCCAGTCACGCAGCAAAAGCTCACAGGCGAACTCA
CCAAAGCCTTTGCTGATGCCATTATGAATGACAAGCAGAAAGCCGAGTTTGCGGTTGAAAAAGAATGCAATTTTGCAATC
TGGCCTAAAGAAATTGGGCGTTTTCGAGTGAATGTGTTTTTACAGCAAGAAAAAATAGGCATGGTGTTGCGTACTATCAC
CACAAAAATACCTAACTTTGAGGATTTAGGCTTACCTAAAGTACTCAAAGAAGTGATGATGACCAAGCGTGGCTTGGTGA
TTTTGGTTGGTGGTACAGGCTCGGGCAAATCCACCACACTGGCTGCGCTGATTGACTACCGCAATGAACACAGCTTCGGT
CATATCATTACAGTAGAAGACCCTGTGGAATATGTGCATCAAAGTAAAAACTGTTTGGTCACTCACCGTGAAGTAGGGCG
TGACACCAACGGCTGGTTCAATGCGCTGAAAAATACATTACGCCAAGCGCCTGATGTTATTTTGATTGGCGAGATTCGCG
ACAGGGAAACCATGGAATTCGCGCTAGCCTTTGCGGAAACCGGACATCTTTGCATGGCAACCTTGCATGCCAATAGCGCC
AATCAAGCGCTTGATCGCATCATCAACTTTTTCCCTGAAGAACGTCATGCGCAACTGCATATGGATTTATCGTTGAATCT
ACGTGCATTTGTGTCACAACGATTAGTTTCGAAAGTCGGTGGCGGCCGCAGTGCGGCTGTTGAGATTCTGCTGAATTCAC
CCTTGATTTCAGATCTGATTTTAAGAGGTGAAACAGGCATGGTAAAAGAGATTATGGCCAAATCAACGGAGCAGGGTATG
CAAACATTCGACCAAGCCTTATTCCAGCTCTGTGAAGACGGTGTCATTTCTGAAGAAGATGCTTTAAGAAATGCCGACTC
ACTCACCGAATTACGTTTGCGCTTTAAACTCAAAGGCAAAAATGCTGGTGCTAAATCAACCAGTATGTTTGCCTTGCATG
AAGAGCCTAAAGAGGAAAAGGCCCCAGAAGTTCAGGCAGCAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DP24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.38

92.268

0.539
  pilU Acinetobacter baylyi ADP1

57.184

89.691

0.513
  pilU Vibrio cholerae strain A1552

54.318

92.526

0.503
  pilT Legionella pneumophila strain ERS1305867

44.545

85.052

0.379
  pilT Legionella pneumophila strain Lp02

44.545

85.052

0.379
  pilT Acinetobacter baumannii strain A118

44.377

84.794

0.376
  pilT Acinetobacter nosocomialis M2

44.377

84.794

0.376
  pilT Acinetobacter baumannii D1279779

44.377

84.794

0.376
  pilT Pseudomonas aeruginosa PAK

44.753

83.505

0.374
  pilT Acinetobacter baylyi ADP1

44

83.763

0.369
  pilT Pseudomonas stutzeri DSM 10701

43.827

83.505

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.683

84.536

0.361
  pilT Vibrio cholerae strain A1552

42.683

84.536

0.361

Multiple sequence alignment