Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FLP15_RS01245 Genome accession   NZ_CP041356
Coordinates   253905..254888 (-) Length   327 a.a.
NCBI ID   WP_142765692.1    Uniprot ID   A0A514Z673
Organism   Lactococcus protaetiae strain KACC 19320     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 248905..259888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLP15_RS01205 (FLP15_01205) - 249047..249889 (-) 843 WP_142765687.1 zinc ABC transporter substrate-binding protein -
  FLP15_RS01210 (FLP15_01210) - 250259..250717 (-) 459 WP_142765688.1 zinc-dependent MarR family transcriptional regulator -
  FLP15_RS01215 (FLP15_01215) comGG 251284..251580 (-) 297 WP_142765689.1 competence type IV pilus minor pilin ComGG Machinery gene
  FLP15_RS01220 (FLP15_01220) comGF 251609..252001 (-) 393 WP_142767397.1 competence type IV pilus minor pilin ComGF Machinery gene
  FLP15_RS01225 (FLP15_01225) comGE 252012..252308 (-) 297 WP_142765690.1 competence type IV pilus minor pilin ComGE Machinery gene
  FLP15_RS01230 (FLP15_01230) comGD 252280..252711 (-) 432 WP_142765691.1 competence type IV pilus minor pilin ComGD Machinery gene
  FLP15_RS01235 (FLP15_01235) comGC 252671..252973 (-) 303 WP_120772581.1 competence type IV pilus major pilin ComGC Machinery gene
  FLP15_RS01240 (FLP15_01240) comGB 252977..253999 (-) 1023 WP_142767398.1 competence type IV pilus assembly protein ComGB Machinery gene
  FLP15_RS01245 (FLP15_01245) comGA 253905..254888 (-) 984 WP_142765692.1 competence type IV pilus ATPase ComGA Machinery gene
  FLP15_RS13680 - 255681..255905 (-) 225 Protein_245 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 37758.22 Da        Isoelectric Point: 6.1914

>NTDB_id=371483 FLP15_RS01245 WP_142765692.1 253905..254888(-) (comGA) [Lactococcus protaetiae strain KACC 19320]
MIQDKASEILTDAMTKESHDIYFIPHQEGYDVYFREGDRRLFFEHLDKELGQAIISHFKFLAGMNTGEKRRTQLGACWYS
LDIGVKRLRMSTVGDFEGRESLVIRILREESRKLEFWFRDEELLASLHCKRGLYLFAGPVGSGKTSLMVELAKKNFMNKQ
VITIEDPVEIVESDFVQLQVNEVIGNDYDELIKCSLRHRPDLLIVGEIRDKKTARAVLRASLTGYTVFSTVHARSISGVW
NRLLELGLSEWELKNSLRRVIYQRLIAGKGVVDIAESEFEKWNARRWNEKMARLFEDGYLTAIEVEAEKVEFDKTSKIDS
VDGKSDC

Nucleotide


Download         Length: 984 bp        

>NTDB_id=371483 FLP15_RS01245 WP_142765692.1 253905..254888(-) (comGA) [Lactococcus protaetiae strain KACC 19320]
ATGATTCAAGATAAGGCAAGCGAAATTTTAACTGATGCGATGACTAAAGAAAGTCATGATATTTATTTTATTCCTCATCA
GGAGGGATACGATGTTTATTTTAGAGAAGGAGACAGGCGTTTATTCTTTGAGCATTTAGACAAGGAATTGGGGCAGGCGA
TAATTTCGCATTTTAAATTTCTCGCTGGGATGAATACAGGGGAGAAACGCAGAACTCAGTTAGGGGCTTGTTGGTACTCG
CTGGACATTGGTGTTAAGCGTTTAAGAATGTCTACGGTTGGAGATTTTGAGGGGAGGGAGTCCTTGGTCATTCGTATTTT
AAGAGAGGAATCAAGGAAGTTGGAGTTTTGGTTTCGGGATGAGGAGCTGTTAGCCAGTCTACATTGTAAGCGAGGATTAT
ATTTGTTTGCGGGTCCAGTAGGTTCAGGAAAAACGTCCTTGATGGTGGAACTTGCAAAGAAGAATTTTATGAATAAACAA
GTAATAACGATTGAAGACCCAGTTGAAATTGTAGAATCTGACTTTGTGCAGTTGCAGGTAAATGAAGTCATCGGTAATGA
TTATGATGAACTCATTAAATGCTCATTGCGTCATCGTCCTGATTTATTAATTGTTGGAGAGATTCGTGATAAAAAGACAG
CACGTGCAGTATTAAGAGCAAGTTTGACTGGTTATACTGTATTTTCTACGGTTCATGCGCGGTCTATTTCGGGAGTGTGG
AATAGACTTTTAGAGCTAGGACTGAGTGAATGGGAATTAAAAAATAGTTTGCGTAGGGTGATTTATCAGCGTCTTATTGC
TGGGAAGGGAGTGGTTGATATTGCAGAAAGTGAATTTGAAAAATGGAATGCCAGGAGATGGAATGAGAAAATGGCGAGAT
TATTTGAAGATGGATATCTTACAGCTATTGAGGTTGAAGCGGAAAAAGTTGAGTTTGATAAAACAAGCAAAATTGATTCA
GTTGATGGCAAATCTGACTGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514Z673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

64.11

99.694

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

56.41

95.413

0.538
  comGA/cglA/cilD Streptococcus pneumoniae D39

56.41

95.413

0.538
  comGA/cglA/cilD Streptococcus pneumoniae R6

56.41

95.413

0.538
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

56.41

95.413

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

55.769

95.413

0.532
  comYA Streptococcus mutans UA159

54.808

95.413

0.523
  comYA Streptococcus mutans UA140

54.487

95.413

0.52
  comYA Streptococcus gordonii str. Challis substr. CH1

54.194

94.801

0.514
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

53.055

95.107

0.505

Multiple sequence alignment